Acinetobacter venetianus, a potential pathogen of red leg disease in freshwater-cultured whiteleg shrimp Penaeus vannamei
Acinetobacter venetianus, a potential pathogen of red leg disease in freshwater-cultured whiteleg shrimp Penaeus vannamei
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DOI：
10.1016/j.aqrep.2020.100543
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发表时间：
2020-11-01
影响因子：
3.7
通讯作者：
Gai, Chunlei
中科院分区：
文献类型：
--
作者：
Huang, Xiaodong;Gu, Yin;Gai, Chunlei
不动杆菌属是近年来在水产养殖中引起大量死亡的病原菌。然而，关于威尼斯不动杆菌作为南美白对虾的潜在病原体的信息很少。在本研究中，A. Venetianus病原体（SHF 1）是淡水养殖白对虾红腿病的病原体。该菌的平均致死剂量（LD_（50））为3.8 × 10 ~（5）CFU/mL ~（-1），并对水培养中使用的酚类、喹诺酮类和四环素类药物产生多重耐药性。此外，致病性A.蛭弧菌粉剂在0.8mg·L ~（-1）时能有效控制威尼斯蛭弧菌，对威尼斯蛭弧菌有明显的抑菌效果，对威尼斯蛭弧菌有较好的保护作用。venetianus攻击在P. vannamei中持续六天。据我们所知，这是第一个确定A。venetianus是淡水养殖白对虾红腿病的潜在病原菌。
The genus Acinetobacter has recently emerged as bacterial pathogens associated with mass mortalities in aqua culture. Yet scarce information is available on Acinetobacter venetianus as a potential pathogen of whiteleg shrimp Penaeus vannamei. In the present study, an A. venetianus pathogen (SHF1) was confirmed as a causative agent of red leg disease in freshwater-cultured whiteleg shrimp. It showed a mean lethal dose (LD50) value of 3.8 x 10(5) CFU mL(-1) and developed multiple resistances to phenicols, quinolones and tetracycline drugs used in aqua culture. In addition, the pathogenic A. venetianus could be effectively controlled by Bdellovibrio powder at 0.8 mg L-1, which exhibited significant antibacterial effect and displayed good protection against A. venetianus challenge in P. vannamei for six days. As far as we know, this is the first study to identify A. venetianus as a potential bacterial pathogen of red leg disease in freshwater-farmed whiteleg shrimp.