Histochemical localization of aldehyde dehydrogenase in the respiratory tract of the Fischer-344 rat.
Histochemical localization of aldehyde dehydrogenase in the respiratory tract of the Fischer-344 rat.
复制标题
Fischer-344 大鼠呼吸道中乙醛脱氢酶的组织化学定位。
DOI：
10.1016/0041-008x(86)90291-7
复制
发表时间：
1986
影响因子：
3.8
通讯作者：
Kevin T. Morgan
中科院分区：
文献类型：
--
作者：
M. Bogdanffy;Holly W. Randall;Kevin T. Morgan
乙醛是一种鼻致癌物和呼吸道刺激物，由醛脱氢酶代谢。醛脱氢酶在Fischer-344大鼠鼻道、气管和肺的单个细胞中的定位使用冷乙二醇甲基丙烯酸酯包埋程序进行组织化学测定。乙醛脱氢酶活性主要在鼻呼吸上皮，特别是在纤毛上皮细胞的核上细胞质中检测到，而嗅上皮几乎没有酶活性。气管上皮细胞表现出很少可检测的醛脱氢酶活性，然而，克拉拉细胞的下细支气管表现出显着的高活性。这些结果证实了以前的生化研究结果，并通过组织化学鉴定鼻呼吸道上皮内特定的乙醛脱氢酶阳性细胞类型来扩展它们。乙醛引起的鼻病变的分布与区域醛脱氢酶缺乏相关，这表明区域对乙醛毒性作用的敏感性可能是由于，至少部分是由于在敏感区域缺乏醛脱氢酶。此外，克拉拉细胞中的醛脱氢酶活性表明渗透到下呼吸道的醛的代谢的可能位点。
Acetaldehyde, a nasal carcinogen and respiratory tract irritant, is metabolized by aldehyde dehydrogenase. The localization of aldehyde dehydrogenase in individual cells of the nasal passages, trachea, and lungs of the Fischer-344 rat was determined histochemically using a cold glycol methacrylate embedding procedure. Aldehyde dehydrogenase activity was detected principally in the nasal respiratory epithelium, especially in the supranuclear cytoplasm of ciliated epithelial cells while olfactory epithelium was almost devoid of enzyme activity. Epithelial cells of the trachea demonstrated little detectable aldehyde dehydrogenase activity, however, the Clara cells of the lower bronchioles showed remarkably high activity. These results corroborate previous biochemical findings and extend them by histochemically identifying particular aldehyde dehydrogenase-positive cell types within the nasal respiratory epithelium. The distribution of nasal lesions induced by acetaldehyde correlated with regional aldehyde dehydrogenase deficiencies suggesting that regional susceptibility to the toxic effects of acetaldehyde may be due, at least in part, to a lack of aldehyde dehydrogenase in the susceptible regions. Furthermore, aldehyde dehydrogenase activity in the Clara cells indicates a possible site for metabolism of aldehydes which penetrate to the lower airways.
正常大鼠肝脏中的过氧化物酶体相关乙醛脱氢酶。
DOI：
10.1016/s0091-3057(80)80018-9
发表时间：
1980
期刊：
Pharmacology, biochemistry, and behavior
影响因子：
--
作者：
Smith,HE;Lindahl,R
通讯作者：
Lindahl,R
双硫仑对大鼠肝脏苯甲醛和乙醛氧化的影响。
影响因子：
5.8
作者：
Hellström-Lindahl,E;Weiner,H
通讯作者：
Weiner,H