Hollow and degradable polyelectrolyte nanocapsules for protein drug delivery.
Hollow and degradable polyelectrolyte nanocapsules for protein drug delivery.
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DOI：
10.1016/j.actbio.2009.06.020
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发表时间：
2010
影响因子：
9.7
通讯作者：
Shujun Shu;Chunyang Sun;Xinge Zhang;Zhongming Wu;Zhen Wang;Chaoxing Li
中科院分区：
文献类型：
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作者：
Shujun Shu;Chunyang Sun;Xinge Zhang;Zhongming Wu;Zhen Wang;Chaoxing Li
通过将水溶性壳聚糖和葡聚糖硫酸盐逐层组装到蛋白质包埋的氨基功能化二氧化硅颗粒上，然后去除二氧化硅，制备了可生物降解的中空胶囊，包裹了蛋白质药物。为了提高封装效率，减少突发释放，我们设计了这个新的系统来实现这两个目标。以牛血清白蛋白(BSA)为模型蛋白，将其包埋在纳米胶囊中。该系统对牛血清白蛋白具有良好的包封率和载药量。在磷酸盐缓冲液中，缓释剂在2小时内释放量降至10%以下。圆二色谱分析表明，与天然BSA相比，释放的BSA没有明显的构象变化。细胞存活率实验表明纳米胶囊具有良好的生物相容性。药物释放动力学机制为Fickian扩散。这些新型的复合纳米胶囊可能为水溶性蛋白质和多肽提供一种很有前途的递送系统。
Biodegradable hollow capsules encapsulating protein drugs were prepared via layer-by-layer assembly of water-soluble chitosan and dextran sulfate on protein-entrapping amino-functionalized silica particles and the subsequent removal of the silica. In order to enhance the encapsulated efficiency and decrease its burst release, we designed this new system to fulfill these two goals. Bovine serum albumin (BSA), which was used as model protein, was entrapped in the nanocapsules. This system demonstrated a good capacity for the encapsulation and loading of BSA. The burst release was decreased to less than 10% in phosphate-buffered saline within 2h. No significant conformation change was noted from the released BSA in comparison with native BSA by using circular dichroism spectroscopy. Cell viability study suggested that the nanocapsules had good biocompatibility. The drug release kinetics mechanism is Fickian diffusion. These kinds of novel composite nanocapsules may offer a promising delivery system for water-soluble proteins and peptides.