EVIDENCE FOR MITOCHONDRIAL ORIGIN OF FROG EGG CYTOPLASMIC DNA
EVIDENCE FOR MITOCHONDRIAL ORIGIN OF FROG EGG CYTOPLASMIC DNA
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蛙卵细胞质 DNA 线粒体来源的证据
DOI：
10.1073/pnas.56.1.269
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发表时间：
1966-01-01
影响因子：
11.1
通讯作者：
DAWID, IB
中科院分区：
文献类型：
--
作者：
DAWID, IB
从尖音蛙和非洲爪蟾两种两栖动物的卵和卵母细胞中提取线粒体DNA。DNA和2种线粒体酶在蔗糖梯度中的平行分布、颗粒内DNA的DNA酶稳定性以及线粒体制备物纯度的电子显微镜检查证明了DNA与线粒体颗粒的缔合。分离DNA并测定其密度;它与先前测定的全卵DNA的密度相同。卵子中至少有三分之二的DNA存在于线粒体中。因此，卵细胞质DNA的大部分是线粒体来源的，并且卵中过量DNA的现象是由每个卵中存在大量线粒体引起的。
DNA was found in purified mitochondria from eggs and oocytes of 2 amphibian species, Rana pipiens and Xenopus laevis. The association of DNA with the mitochondrial particles was demonstrated by the parallel distribution of DNA and 2 mitochondrial enzymes in sucrose gradients, by the DNase-stability of the DNA inside the particles, and by electron microscopic checks of the purity of the mitochondrial preparations. The DNA was isolated and its density determined; it is identical to the density of whole-egg DNA determined previously. At least two thirds of the DNA of eggs was found in the mitochondria. The bulk of egg cytoplasmic DNA is thus of mitochondrial origin and the phenomenon of excess DNA in eggs is caused by the presence of a large number of mitochondria per egg.