CLONING OF AN AVERMECTIN-SENSITIVE GLUTAMATE-GATED CHLORIDE CHANNEL FROM CAENORHABDITIS-ELEGANS
CLONING OF AN AVERMECTIN-SENSITIVE GLUTAMATE-GATED CHLORIDE CHANNEL FROM CAENORHABDITIS-ELEGANS
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DOI：
10.1038/371707a0
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发表时间：
1994-10-20
期刊：
影响因子：
64.8
通讯作者：
ARENA, JP
中科院分区：
文献类型：
--
作者：
CULLY, DF;VASSILATIS, DK;ARENA, JP
阿维菌素是一类用于防治线虫和节肢动物寄生虫的大环内酯类化合物(1)。伊维菌素(22，23-二氢阿维菌素B-1a)在兽医中被广泛用作驱虫剂，并用于治疗人类的盘尾丝虫病或河盲症(1，2)。阿维菌素(阿维菌素B-1a)是一种用于作物保护的杀螨剂和杀虫剂(1)。阿维菌素与脊椎动物和无脊椎动物的GABA受体(3-7)和无脊椎动物的谷氨酸门控氯离子通道(8-11)相互作用。土壤线虫秀丽线虫是研究阿维菌素作用机理的有效模型(11-15)。在非洲爪哇卵母细胞中表达的线虫信使RNA编码阿维菌素敏感的谷氨酸门控氯通道(11，14)。为了阐明该通道的结构和性质，我们使用非洲爪哇卵母细胞表达克隆了编码阿维菌素敏感的谷氨酸门控氯离子通道的两个功能互补的DNA。我们发现这些蛋白的电生理和结构性质表明它们是配体门控离子通道超家族的新成员。
THE avermectins are a family of macrocyclic lactones used in the control of nematode and arthropod parasites(1). Ivermectin (22,23-dihydroavermectin B-1a) is widely used as an anthelmintic in veterinary medicine and is used to treat onchocerciasis or river blindness in humans(1,2). Abamectin (avermectin B-1a) is a miticide and insecticide used in crop protection(1). Avermectins interact with vertebrate and invertebrate GABA receptors(3-7) and invertebrate glutamate-gated chloride channels(8-11). The soil nematode Caenorhabditis elegans has served as a useful model to study the mechanism of action of avermectins(11-15). A C. elegans messenger RNA expressed in Xenopus oocytes encodes an avermectin-sensitive glutamate-gated chloride channel(11,14). To elucidate the structure and properties of this channel, we used Xenopus oocytes for expression cloning of two functional complementary DNAs encoding an avermectin-sensitive glutamate-gated chloride channel. We find that the electrophysiological and structural properties of these proteins indicate that they are new members of the ligand-gated ion channel superfamily.