LONG-TERM STORAGE OF EIGHT-CELL MOUSE EMBRYOS AT MINUS 196 CELSIUS
LONG-TERM STORAGE OF EIGHT-CELL MOUSE EMBRYOS AT MINUS 196 CELSIUS
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DOI：
10.1007/bf01131376
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
LYON M F
中科院分区：
文献类型：
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作者：
GLENISTER P H;LYON M F
突变小鼠的种群已经从储存在液氮中长达11年的不同时期的八细胞胚胎中重建，并且没有发现存活率随储存时间而恶化的证据。此外，对储存期间背景辐射的模拟累积效应的研究未能发现胚胎暴露于相当于约2000年背景辐射的任何有害影响。然而，在某些情况下，携带突变基因或染色体异常的胚胎在冷冻和解冻过程中的存活率往往低于F1杂交胚胎。虽然这种影响可能是独立的存储时间，最近的技术改进后，胚胎生存是值得欢迎的。
Stocks of mutant mice have been reestablished from eight-cell embryos stored in liquid nitrogen for varying periods up to 11 years, and no evidence has been found of deterioration of survival with time of storage. Also, studies on the simulated cumulative effect of background radiation during storage failed to find any detrimental effect when embryos were exposed to the equivalent of about 2000 years of background radiation. However, in some cases embryos that carry mutant genes or chromosome anomalies tend to survive the freezing and thawing procedure less well than F1 hybrid embryos. Although this effect is probably independent of storage time, recent improvements in technique upon embyronic survival are to be welcomed.