Glucose repression of fbp1 transcription of Schizosaccharomyces pombe is partially regulated by adenylate cyclase activation by a G protein alpha subunit encoded by gpa2 (git8).
Glucose repression of fbp1 transcription of Schizosaccharomyces pombe is partially regulated by adenylate cyclase activation by a G protein alpha subunit encoded by gpa2 (git8).
复制标题
粟酒裂殖酵母 fbp1 转录的葡萄糖抑制部分受到 gpa2 (git8) 编码的 G 蛋白 α 亚基激活的腺苷酸环化酶的调节。
DOI：
10.1093/genetics/138.1.39
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Hoffman,CS
中科院分区：
文献类型：
--
作者：
Nocero,M;Isshiki,T;Yamamoto,M;Hoffman,CS
在分裂酵母裂糖酵母（Schizosaccharomyces pombe）中，遗传学研究已经确定了葡萄糖抑制fbp1转录所需的基因。git2基因，也被称为cyr1，编码腺苷酸环化酶。腺苷酸环化酶将ATP转化为第二信使cAMP，作为许多真核生物信号转导途径的一部分。git1、git3、git5、git7、git8和git10基因作用于腺苷酸环化酶上游，可能编码腺苷酸环化酶激活途径。在哺乳动物细胞中，腺苷酸环化酶的酶活性由异三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）调节。在出芽酵母中，腺苷酸环化酶的酶活性是由鸟嘌呤核苷酸结合的Ras蛋白调控的。我们在这里表明，git8与编码与G蛋白α亚基同源的蛋白质的gpa2基因相同。gpa2+在高拷贝数下抑制另外两个基因git3和git5的突变。此外，git3或git5的突变在gpa2 （git8）缺失的菌株中具有加性效应，因为它显著增加了fbp1-lacZ报告基因的表达。因此，git3和git5似乎与gpa2 （git8）协同或独立作用来调节腺苷酸环化酶活性。
In the fission yeast Schizosaccharomyces pombe, genetic studies have identified genes that are required for glucose repression of fbp1 transcription. The git2 gene, also known as cyr1, encodes adenylate cyclase. Adenylate cyclase converts ATP into the second messenger cAMP as part of many eukaryotic signal transduction pathways. The git1, git3, git5, git7, git8 and git10 genes act upstream of adenylate cyclase, presumably encoding an adenylate cyclase activation pathway. In mammalian cells, adenylate cyclase enzymatic activity is regulated by heterotrimeric guanine nucleotide-binding proteins (G proteins). In the budding yeast Saccharomyces cerevisiae, adenylate cyclase enzymatic activity is regulated by monomeric, guanine nucleotide-binding Ras proteins. We show here that git8 is identical to the gpa2 gene that encodes a protein homologous to the alpha subunit of a G protein. Mutations in two additional genes, git3 and git5 are suppressed by gpa2+ in high copy number. Furthermore, a mutation in either git3 or git5 has an additive effect in strains deleted for gpa2 (git8), as it significantly increases expression of an fbp1-lacZ reporter gene. Therefore, git3 and git5 appear to act either in concert with or independently from gpa2 (git8) to regulate adenylate cyclase activity.