Human coronavirus NL63 and 229E seroconversion in children
Human coronavirus NL63 and 229E seroconversion in children
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DOI：
10.1128/jcm.00533-08
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发表时间：
2008-07-01
影响因子：
9.4
通讯作者：
van der Hoek, Lia
中科院分区：
文献类型：
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作者：
Dijkman, Ronald;Jebbink, Maarten F.;van der Hoek, Lia
2004年，确定了新型呼吸道人冠状病毒NL63（HCOV-NL63），随后的研究表明该病毒已在全球范围内传播。 HCOV-229E是HCOV-NL63的近亲，两种病毒感染都可以导致幼儿住院，免疫功能低下的人和老年人。感染了HCOV-NL63的儿童通常会出现臀部，气道阻塞。在这项研究中，我们调查了哪个年龄儿童首次面对HCOV-NL63感染，因此，在哪个年龄时，他们将血清convert降低到HCOV-NL63阳性。我们设计了一个重组HCOV-229E和重组HCOV-NL63核素蛋白蛋白酶连接的免疫吸附测定法，并对纵向和横截面血清样品进行了血清ePIDEMIology调查。从13个新生儿收集了纵向血清样品，这些新生儿的数据从至少18个月的多个时间点获得。对于横断面调查，我们测试了139名儿童的血清样本，包括16岁儿童的新生儿。在检查纵向血清样品的检查中，我们观察到所有儿童在出生时出生时患有母体抗NL63和抗229E抗体，在3个月内消失了。在随访期间，13名儿童中有7个变成了HCOV-NL63血清阳性，而只有2名HCOV-229E血清阳性。横截面血清样品的血清学数据表明，年龄组2.5至3.5岁的儿童中有75％和65％分别为HCOV-NL63和HCOV-229E血清蛋白阳性。我们得出的结论是，平均而言，HCOV-NL63和HCOV-229E血清转化发生在儿童年龄3.5岁之前。
In 2004, the novel respiratory human coronavirus NL63 (HCoV-NL63) was identified, and subsequent research revealed that the virus has spread worldwide. HCoV-229E is a close relative of HCoV-NL63, and infection with either virus can lead to the hospitalization of young children, immunocompromised persons, and the elderly. Children infected with HCoV-NL63 often develop croup, with obstruction of the airway. In this study we investigated at which age children are confronted for the first time with an HCoV-NL63 infection and, thus, at which age they seroconvert to HCoV-NL63 positivity. We designed a recombinant HCoV-229E and a recombinant HCoV-NL63 nucleocapsid protein enzyme-linked immunosorbent assay and performed a seroepidemiology survey on longitudinal and cross-sectional serum samples. The longitudinal serum samples were collected from 13 newborns, and data for those newborns were available from multiple time points spanning a period of at least 18 months. For the cross-sectional survey we tested serum samples of 139 children, including newborns to children 16 years of age. In examinations of the longitudinal serum samples we observed that all of the children had maternal anti-NL63 and anti-229E antibodies at birth that disappeared within 3 months. Seven of the 13 children became HCoV-NL63 seropositive during follow-up, whereas only 2 became HCoV-229E seropositive. The serology data of the cross-sectional serum samples revealed that 75% and 65% of the children in the age group 2.5 to 3.5 years were HCoV-NL63 and HCoV-229E seropositive, respectively. We conclude that on average, HCoV-NL63 and HCoV-229E seroconversion occurs before children reach the age of 3.5 years.