The effect of gene drive on containment of transgenic mosquitoes
The effect of gene drive on containment of transgenic mosquitoes
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DOI：
10.1016/j.jtbi.2009.01.031
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发表时间：
2009-05-21
影响因子：
2
通讯作者：
Marshall, John M.
中科院分区：
文献类型：
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作者：
Marshall, John M.
疟疾和登革热等蚊媒疾病仍然是世界大部分地区的主要健康问题。几种潜在的疾病控制新方法利用基因驱动将抗病原体基因传播到蚊子种群中。在发布之前，这些项目将需要在户外笼子里进行试验，转基因蚊子可以从那里逃脱，尽管数量很少。大多数少量导入的基因很可能会从环境中消失；然而，基因驱动机制增强了导入基因的侵袭性。因此，在意外释放后，引入的转基因可能比普通基因更有可能持续存在。在这里，我们建立了随机模型来分析几种基因驱动机制的丢失概率，包括归巢内切酶基因、转座元件、Medea元件、胞内细菌Wolbachia、工程欠优势基因和减数分裂驱动。我们发现，对于目前正在考虑用于控制蚊媒疾病的六种基因驱动系统，美狄亚和沃尔巴克氏杆菌代表了侵袭性和遏制之间的最佳折衷。爱思唯尔有限公司出版。
Mosquito-borne diseases such as malaria and dengue fever continue to be a major health problem through Much of the world. Several new potential approaches to disease control utilize gene drive to spread anti-pathogen genes into the mosquito Population. Prior to a release, these projects will require trials in outdoor cages from which transgenic mosquitoes may escape, albeit in small numbers. Most genes introduced in small numbers are very likely to be lost from the environment; however, gene drive mechanisms enhance the invasiveness of introduced genes. Consequently, introduced transgenes may be more likely to persist than ordinary genes following an accidental release. Here, we develop stochastic models to analyze the loss probabilities for several gene drive mechanisms, including homing endonuclease genes, transposable elements, Medea elements, the intracellular bacterium Wolbachia, engineered underdominance genes, and meiotic drive. We find that Medea and Wolbachia present the best compromise between invasiveness and containment for the six gene drive systems Currently being considered for the control of mosquito-borne disease. Published by Elsevier Ltd.