Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni) on alloxan-induced diabetic rats
Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni) on alloxan-induced diabetic rats
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DOI：
10.4103/0975-7406.80779
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发表时间：
2011-04-01
影响因子：
0.8
通讯作者：
Jain, D. C.
中科院分区：
文献类型：
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作者：
Misra, Himanshu;Soni, Manish;Jain, D. C.
目的：研究菊科甜菊叶中极性(苯：丙酮，1：1，v/v)提取物对四氧嘧啶诱导的糖尿病大鼠的治疗作用。材料和方法：成年Wistar大鼠腹腔注射糖尿病模型。注射四氧嘧啶(180 mg/kg)。中极性提取物按200 mg/kg体重和400 mg/kg体重每日灌胃，连续10天。对照组给予0.9%生理盐水，持续时间相同。以格列本脲作为甜菊提取物的阳性对照参照物。结果：与标准阳性对照药物格列本脲相比，雷公藤中极叶提取物(200和400 mg/kg)能延缓而显著地降低血糖(P<；0.01)，且治疗后不出现低血糖，体重减轻。结论：使用磺脲类药物(格列本脲)治疗糖尿病会导致低血糖，随后体重明显减轻，这是这些药物最令人担忧的影响。甜叶菊提取物可拮抗四氧嘧啶的坏死作用，从而对胰腺细胞有恢复活力的作用。
Objective: To investigate the medicative effects of medium-polar (benzene:acetone, 1:1, v/v) extract of leaves from Stevia rebaudiana (family Asteraceae) on alloxan-induced diabetic rats. Materials and Methods: Diabetes was induced in adult albino Wistar rats by intraperitoneal (i.p.) injection of alloxan (180 mg/kg). Medium-polar extract was administered orally at daily dose of 200 and 400 mg/kg body wt. basis for 10 days. The control group received normal saline (0.9%) for the same duration. Glibenclamide was used as positive control reference drug against Stevia extract. Results: Medium-polar leaf extract of S. rebaudiana (200 and 400 mg/kg) produced a delayed but significant (P < 0.01) decrease in the blood glucose level, without producing condition of hypoglycemia after treatment, together with lesser loss in the body weight as compared with standard positive control drug glibenclamide. Conclusions: Treatment of diabetes with sulfonylurea drugs (glibenclamide) causes hypoglycemia followed by greater reduction in body weight, which are the most worrisome effects of these drugs. Stevia extract was found to antagonize the necrotic action of alloxan and thus had a re-vitalizing effect on -cells of pancreas.