Epidermal growth factor inhibits growth while increasing the expression of an ecto-Ca2+-ATPase of a human hepatoma cell line.
Epidermal growth factor inhibits growth while increasing the expression of an ecto-Ca2+-ATPase of a human hepatoma cell line.
复制标题
表皮生长因子抑制生长，同时增加人肝癌细胞系的 ecto-Ca2 -ATPase 的表达。
DOI：
10.1016/0006-291x(85)90564-9
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发表时间：
1985
影响因子：
3.1
通讯作者：
Villela,J
中科院分区：
文献类型：
--
作者：
Knowles,AF;Salas-Prato,M;Villela,J
We have obtained a cloned cell line (Li-7A) from primary cultures of a human hepatoma xenograft (Li-7). Li-7A was able to grow in the absence of serum. Growth was stimulated 0–3 fold by addition of newborn calf serum, but was inhibited in DME F12 media containing nine growth factors. The ectoMg 2+-ATPase was 1.5–2 fold higher than the ectoCa 2+-ATPase activity in cells grown in media with or without serum. In cells grown in media supplemented with the nine factors, the ectoCa 2+-ATPase activity exceeded the ectoMg 2+-ATPase, and there was also a 5–10 fold increase in its specific activity. Inhibition of growth was due to epidermal growth factor alone. The increased expression of the ecto-Ca 2+-ATPase was absolutely dependent on EGF, but also required hydrocortisone and cholera toxin. The characteristics of Li-7A cells make it a suitable system for studying both the mechanism of action of EGF and plasma membrane ATPases.
