Emergence of fluoroquinolone resistance in the native Campylobacter coli population of pigs exposed to enrofloxacin
Emergence of fluoroquinolone resistance in the native Campylobacter coli population of pigs exposed to enrofloxacin
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DOI：
10.1093/jac/dkh150
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发表时间：
2004-05-01
影响因子：
5.2
通讯作者：
Roe, JM
中科院分区：
文献类型：
--
作者：
Delsol, AA;Sunderland, J;Roe, JM
目的：研究恩诺沙星单用5天对5周龄常规断奶仔猪结肠弯曲菌群的影响。材料：将12头猪分为两组，一组给予恩诺沙星治疗剂量(15 mg/头/天)，另一组不给予恩诺沙星治疗作为对照。从粪便标本中分离出结肠弯曲杆菌，通过测定MIC值进行环丙沙星耐药性检测。通过测序和变性高效液相色谱鉴定耐药株gyrA基因的喹诺酮类药物耐药决定区(QRDR)突变。用高效毛细管电泳法检测猪粪便中恩诺沙星及其主要代谢物环丙沙星的含量。结果：所有猪在治疗前均未检出耐喹诺酮类大肠埃希菌(n=867)。
Objective: The effect of a single 5 day enrofloxacin treatment on the native Campylobacter coli population in conventionally weaned 5-week-old pigs was investigated.Materials: Twelve pigs were split into two groups of six: one group was treated with a therapeutic dose (15 mg/pig/day) of enrofloxacin and the other remained untreated to act as the control. Campylobacter coli were isolated from faecal samples and tested for ciprofloxacin resistance by measuring MIC values. Mutations in the quinolone resistance-determining region (QRDR) of the gyrA gene of resistant isolates were identified by sequencing and denaturing HPLC. Levels of enrofloxacin and its primary metabolite ciprofloxacin in the pig faeces were also measured by HPLC.Results: No quinolone-resistant C. coli (n = 867) were detected in any of the pigs prior to treatment, indicating