Hepatocyte growth factor activator: a possible regulator of morphogenesis during fetal development of the rat gastrointestinal tract.
Hepatocyte growth factor activator: a possible regulator of morphogenesis during fetal development of the rat gastrointestinal tract.
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肝细胞生长因子激活剂：大鼠胃肠道胎儿发育过程中形态发生的可能调节剂。
DOI：
10.1006/bbrc.1998.9808
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发表时间：
1998
影响因子：
3.1
通讯作者：
H. Fukamachi
中科院分区：
文献类型：
--
作者：
Y. Matsubara;M. Ichinose;N. Yahagi;S. Tsukada;M. Oka;K. Miki;S. Kimura;M. Omata;K. Shiokawa;N. Kitamura;Y. Kaneko;H. Fukamachi
本文研究了肝细胞生长因子激活剂(HGFA)在16~21天胎鼠胃肠道形态发生中的作用。通过我们最近建立的使用螯合和解剖显微镜的方法，样品可以分离出上皮和间质，基本上没有交叉污染。RT-PCR法检测HGFA基因、肝细胞生长因子(HGF)及其受体c-MET基因在各组织元素中的表达。在胎鼠胃肠道中，HGFA基因的mRNA信号仅在表达c-met mRNA的上皮细胞中观察到。相反，HGF mRNA的表达仅限于间质成分，表明胃肠道存在局部的HGF系统；HGF的非活性形式(ProHGF)从间质分泌，然后被靶细胞分泌的HGFA切割成活性形式。在胃肠道的形态发生和组织分化过程中，HGF及其受体/c-met基因的表达明显增强，HGFA基因表达水平在整个胃肠道均升高，但在前胃几乎检测不到HGFA基因的表达。这些结果有力地表明，胃肠道的形态发生不仅受到HGF的局部增加的调节，而且还受到ProHGF蛋白分解活性的增强的调节。因此，局部合成的HGFA可能在胃肠道发育过程中对HGF的形态发生作用起着重要的调节作用。
The role played by the hepatocyte growth factor activator (HGFA) during morphogenesis of the gastrointestinal tract was investigated in fetal rats between days 16 and 21 of gestation. By our recently established method using chelation and dissecting microscope, samples could be separated into epithelium and mesenchyme, essentially without cross-contamination. The expression of the gene for HGFA together with those for hepatocyte growth factor (HGF) and its receptor, c-met, was investigated in each tissue element by RT-PCR. In the fetal rat gastrointestinal tract, mRNA signals for the HGFA gene were observed only in epithelia expressing c-met mRNA. In contrast, expression of HGF mRNA was limited to the mesenchymal elements, indicating the presence of a local HGF system in the gastrointestinal tract; an inactive form of HGF (proHGF) is secreted from the mesenchyme and then cleaved into the active form by HGFA secreted by the target epithelia. During the period of morphogenesis and histodifferentiation in the gastrointestinal tract, enhanced expression of the genes for HGF and its receptor/c-met was evident, with elevated HGFA mRNA level observed throughout the gastrointestinal tract except in the forestomach, where mRNA expression was barely detectable. These results strongly suggest the possibility that morphogenesis of the gastrointestinal tract is regulated not only by a local increase in production of HGF, but also by enhanced proteolytic activation of proHGF. Thus, it is probable that locally synthesized HGFA plays a significant role as a regulator of the morphogenic action of HGF during gastrointestinal tract development.
DOI：
--
发表时间：
1993-09
期刊：
The American journal of pathology
影响因子：
--
作者：
W. Mars;R. Zarnegar;G. Michalopoulos
通讯作者：
W. Mars;R. Zarnegar;G. Michalopoulos
DOI：
10.1073/pnas.87.7.2725
发表时间：
1990-04-01
影响因子：
11.1
作者：
GILLILAND, G;PERRIN, S;BUNN, HF
通讯作者：
BUNN, HF