Mineralized DNA-collagen complex-based biomaterials for bone tissue engineering.
Mineralized DNA-collagen complex-based biomaterials for bone tissue engineering.
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DOI：
10.1016/j.ijbiomac.2020.06.126
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发表时间：
2020-06
影响因子：
8.2
通讯作者：
Bryan D. James;Paxton Guerin;Zion Iverson;J. Allen
中科院分区：
文献类型：
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作者：
Bryan D. James;Paxton Guerin;Zion Iverson;J. Allen
DNA 是一种高度聚阴离子的生物分子，可与胶原蛋白和羟基磷灰石复合。通过结合这些复合物，我们合成了用羟基磷灰石矿化的核酸胶原复合物（NACC）。该复合物是使用短的单分散单链 DNA、I 型胶原蛋白和矿化介质制成的。它们快速自组装成矿化 NACC 微纤维和 3D NACC 凝胶。在纳米尺度上，这些复合物是分层的、交织的、卷曲的纳米原纤维，类似于天然的细胞外基质，其矿化了互穿的纳米晶羟基磷灰石相。矿化可以在 NACC 形成之前或之后完成，从而能够对过程进行时间控制。响应 NACC 材料，原代人成骨细胞呈现骨细胞样形态。此外，细胞在 3 天内聚集并将 NACC 凝胶重塑为致密的组织样结构。 NACC 纤维和凝胶不仅有望作为骨传导涂层和支架，而且可以作为使用这种新型天然生物材料的任何组织的涂层和支架。
DNA is a highly polyanionic biomolecule that complexes with both collagen and hydroxyapatite. By combining these complexes, we synthesized nucleic-acid collagen complexes (NACC) mineralized with hydroxyapatite. The composite complexes were made using a short, monodisperse single-stranded DNA, type I collagen, and mineralizing medium. They rapidly self-assembled into both mineralized NACC microfibers and 3D NACC gels. At the nanoscale, these complexes are hierarchical, interwoven, curly nanofibrils resembling native extracellular matrix, which mineralized an interpenetrating nanocrystalline hydroxyapatite phase. Mineralization was able to be done either before or after NACC formation enabling temporal control of the process. In response to the NACC material, primary human osteoblasts took on an osteocyte-like morphology. Moreover, the cells agglomerated and remodeled the NACC gels into densified, tissue-like structures within 3 days. NACC fibers and gels have promise not only as osteoconductive coatings and scaffolds, but as coatings and scaffolds for any tissue using this new form of naturally-derived biomaterials.