The role of selenium in insulin-like growth factor I receptor (IGF-IR) expression and regulation of apoptosis in mouse osteoblasts.
The role of selenium in insulin-like growth factor I receptor (IGF-IR) expression and regulation of apoptosis in mouse osteoblasts.
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DOI：
10.1016/j.chemosphere.2015.11.003
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发表时间：
2016-02
期刊：
影响因子：
8.8
通讯作者：
G. Ren;T. Ali;Wei Chen;Dandan Han;Limei Zhang;Xiaolong Gu;Shiyao Zhang;Laidi Ding;S. Fanning;B. Han
中科院分区：
文献类型：
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作者：
G. Ren;T. Ali;Wei Chen;Dandan Han;Limei Zhang;Xiaolong Gu;Shiyao Zhang;Laidi Ding;S. Fanning;B. Han
硒（Se）是动物和人类的必需成分。硒通过调节细胞周期、刺激细胞凋亡和激活某些细胞因子等发挥化学保护作用，这是众所周知的。然而，Se对IGF-IR表达的综合影响及其对细胞凋亡的调节尚未被研究。因此，本研究的目的是报告不同浓度的硒对小鼠体重、血清IGF-IR水平和组织病理学的影响；体内实验显示，给予规定浓度的硒 30 天后，体重、血清 IGF-IR 水平显着下降，并对肝脏、肾脏、心脏和脾脏产生显着的毒性作用。然而，中等浓度（0.1 mg/kg）的 Se 逐渐改善体重和血清 IGF-IR。在体外成骨细胞实验中表明，在 5 × 10−6 和 10−5mol/L Se 浓度下，与对照细胞相比，MTT 活性降低。细胞周期、TEM 和 caspase-3 活性支持了这些观察结果，包括成骨细胞亚 G1 期的增加和显着的细胞凋亡，以及 IGF-IR mRNA 表达和蛋白质水平的降低。此外，与非siRNA组相比，siRNA组的成骨细胞中MTT活性、IGF-IR mRNA和蛋白水平降低，caspase-3活性升高。这些数据表明 Se 显着影响 IGF-IR 表达，并且有助于成骨细胞的增殖和细胞凋亡的调节。
Selenium (Se) is an essential component for animals and human beings. The chemoprotective role of Se,viathe regulation of the cell cycle, stimulation of apoptosis and activation of some cytokines among others, is well known; however, the comprehensive effects of Se on the expression of IGF-IR and its regulation of apoptosis have not been investigated. Thus the aim of this study was to report on the effects that different concentrations of Se extert on body weight, blood serum IGF-IR levels and histopathology in mice; and on IGF-IR expression, proliferation and apoptosis in mouse osteoblasts.In vivoexperiments showed a significant decrease in body weight, serum levels of IGF-IR and prominent toxicant effects on the liver, kidney, heart and spleen following the administration of defined concentrations of Se for 30 d. However, moderate levels (0.1 mg/kg) of Se gradually improved weight and serum IGF-IR.In vitroosteoblast experiments revealed that at concentrations of 5 × 10−6and 10−5mol/L Se, MTT activity decreased in comparison with control cells. Cell cycle, TEM and caspase-3 activity supported these observations including an increase in the sub-G1 phase and notable apoptosis in osteoblasts, along with a decrease in the expression of mRNA and protein levels of IGF-IR. Moreover, the MTT activity, mRNA and protein levels of IGF-IR in osteoblasts were decreased and caspase-3 activity was increased in siRNA groups as compared with non-siRNA groups. These data suggest that Se significantly affects IGF-IR expression, and that it contributes to the proliferation and regulation of apoptosis in osteoblasts.