Effects of vitamin E and selenium on immune responses of peripheral blood, colostrum, and milk leukocytes of sows.
Effects of vitamin E and selenium on immune responses of peripheral blood, colostrum, and milk leukocytes of sows.
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DOI：
10.2527/1993.7192464x
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发表时间：
1993-09
影响因子：
3.3
通讯作者：
H. Wuryastuti;H. D. Stowe;R. W. Bull;E. R. Miller
中科院分区：
文献类型：
--
作者：
H. Wuryastuti;H. D. Stowe;R. W. Bull;E. R. Miller
本研究旨在评估日粮中维生素 E (E) 和（或）硒 (Se) 浓度如何影响妊娠和围产期母猪的免疫反应。经产母猪 (24 头)，在配种时被分配到四组之一，在妊娠期间和哺乳期至哺乳期第 4 天，饲喂以青贮、带壳玉米豆粕为主的日粮，不添加 E 或 Se (-E-Se)，添加 0.3 mg Se/kg (-E+Se)，添加 60 IU E/kg (+E-Se)，或同时添加 E 和 Se (+E+Se)。在妊娠第 0、30、60 和 90 天以及分娩时采集血液进行血清 E 和 Se 测定。从血液、初乳和 4 天牛奶样本中分离淋巴细胞和多形核细胞 (PMN)，用于免疫研究。与对照（+E+Se）饮食相比，-E-Se饮食降低（P < .05）血清生育酚和硒浓度，降低外周血（PBL）和初乳（CL）淋巴细胞的有丝分裂反应，血液和初乳PMN的吞噬活性，以及血液、初乳和乳汁PMN的杀菌活性。 -E+Se 饮食降低 (P < .05) 血清生育酚浓度、PBL 和 CL 的有丝分裂反应以及 PBL 的吞噬活性。 +E-Se 饮食降低 (P < .05) 血清硒浓度和 PMN 的吞噬活性。数据表明，E 限制会抑制 PBL 和 PMN 免疫功能，而 Se 限制则主要抑制 PMN 功能。
This study was designed to assess how dietary vitamin E (E) and (or) selenium (Se) concentrations affect immune responses of gestating and peripartum sows. Multiparous sows (24), assigned to one of four groups at breeding, were fed ensiled, shelled corn-soybean meal-based diets without supplemental E or Se (-E-Se), with .3 mg of Se/kg (-E+Se), with 60 IU of E/kg (+E-Se), or with both supplemental E and Se (+E+Se) during gestation and to d 4 of lactation. Blood was obtained on 0, 30, 60, and 90 d of gestation and at parturition for serum E and Se assays. Lymphocytes and polymorphonuclear cells (PMN) were isolated from the blood, colostrum, and 4-d milk samples for immune studies. Compared with the control (+E+Se) diet, the -E-Se diet reduced (P < .05) the serum tocopherol and Se concentrations, the mitogenic responses of lymphocytes of peripheral blood (PBL) and colostrum (CL), the phagocytic activity of blood and colostral PMN, and the microbicidal activity of blood, colostral, and milk PMN. The -E+Se diet reduced (P < .05) the serum tocopherol concentrations, the mitogenic responses of PBL and CL, and the phagocytic activity of PBL. The +E-Se diet reduced (P < .05) serum Se concentrations and the phagocytic activity of PMN. The data indicated that E restriction depressed PBL and PMN immune functions, whereas Se restriction depressed mainly PMN function.