Phenotypic resistance in mycobacteria: is it because I am old or fat that I resist you?
Phenotypic resistance in mycobacteria: is it because I am old or fat that I resist you?
复制标题
DOI：
10.1093/jac/dkv178
复制
发表时间：
2015-10-01
影响因子：
5.2
通讯作者：
Gillespie, Stephen H.
中科院分区：
文献类型：
--
作者：
Hammond, Robert J. H.;Baron, Vincent O.;Gillespie, Stephen H.
目的：我们的目的是探讨抗分枝杆菌抗生素的表型耐药现象，并确定这是否与细胞年龄或存在的lipidbodies.Methods：积累的脂质体阳性[脂质丰富（LR）]细胞，随后使用细胞染色和流式细胞术。将耻垢分枝杆菌、海分枝杆菌、偶发分枝杆菌和牛分枝杆菌（BCG）的LR细胞与非含脂体的[脂质贫乏（LP）]细胞分离，并测定其MBC。结果：所有种属的LR细胞对抗生素的耐药性均高于LP细胞。对于BCG，敏感率如下：利福平，5倍;异烟肼，16.7倍;乙胺丁醇，5倍;环丙沙星，5倍。表型耐药被发现在LR细胞无论细胞age.Conclusions：我们已经表明，表型抗生素耐药性与脂质体的存在无关的细胞年龄。这些数据对我们理解分枝杆菌感染的复发具有重要意义。
Objectives: We aimed to explore the phenomenon of phenotypic resistance to antimycobacterial antibiotics and to determine whether this was associated with cell age or the presence of lipid bodies.Methods: The accumulation of lipid-body-positive [lipid-rich (LR)] cells was followed using cell staining and flow cytometry. LR cells of Mycobacterium smegmatis, Mycobacterium marinum, Mycobacterium fortuitum and Mycobacterium bovis (BCG) were separated from non-lipid-body-containing [lipid-poor (LP)] cells and their MBCs determined. We also compared the MBCs for LR and LP cells from 'old' and 'young' cultures.Results: The LR cells of all species were more resistant to antibiotics than LP cells. For BCG, the susceptibility ratios were as follows: rifampicin, 5x; isoniazid, 16.7x; ethambutol, 5x; and ciprofloxacin, 5x. Phenotypic resistance was found in LR cells irrespective of cell age.Conclusions: We have shown that phenotypic antibiotic resistance is associated with the presence of lipid bodies irrespective of cell age. These data have important implications for our understanding of relapse in mycobacterial infections.