Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology
Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology
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DOI：
10.1038/s41596-020-0300-1
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发表时间：
2020-04-01
期刊：
影响因子：
14.8
通讯作者：
Zhao, Yongxin
中科院分区：
文献类型：
--
作者：
Bucur, Octavian;Fu, Feifei;Zhao, Yongxin
在病理学中，显微镜是分析人体组织的重要工具，既用于疾病状态的科学研究，也用于诊断。然而，病理中常用的显微镜受到衍射的限制，分辨率受到限制。最近，我们发现，通过一种化学过程，可以将保存的细胞和组织在线性维度上各向同性地扩张4-5倍。我们称这一过程为扩张显微镜(EXM)。ExM可以在传统显微镜上实现纳米级分辨率成像。在这里，我们描述了各种人体组织和临床标本的简单而有效的物理扩张方案，包括石蜡包埋的、新鲜的冷冻和化学染色的人体组织。这些方案只需要廉价的、商业上可用的试剂和常规病理实验室中常见的硬件。我们的方案是为在传统荧光显微镜方面经验丰富的研究人员和病理学家编写的。常规的常规方法，即扩张病理学，可以在免疫组织切片1d和未染色标本的2d内完成。我们还包括一种新的、快速变化的、快速扩张的病理，可以在
In pathology, microscopy is an important tool for the analysis of human tissues, both for the scientific study of disease states and for diagnosis. However, the microscopes commonly used in pathology are limited in resolution by diffraction. Recently, we discovered that it was possible, through a chemical process, to isotropically expand preserved cells and tissues by 4-5x in linear dimension. We call this process expansion microscopy (ExM). ExM enables nanoscale resolution imaging on conventional microscopes. Here we describe protocols for the simple and effective physical expansion of a variety of human tissues and clinical specimens, including paraffin-embedded, fresh frozen and chemically stained human tissues. These protocols require only inexpensive, commercially available reagents and hardware commonly found in a routine pathology laboratory. Our protocols are written for researchers and pathologists experienced in conventional fluorescence microscopy. The conventional protocol, expansion pathology, can be completed in similar to 1 d with immunostained tissue sections and 2 d with unstained specimens. We also include a new, fast variant, rapid expansion pathology, that can be performed on