Expression of thyroid transcription factor-1 in normal and neoplastic lung tissues
Expression of thyroid transcription factor-1 in normal and neoplastic lung tissues
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DOI：
10.1097/01.mp.0000028572.44247.cf
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发表时间：
2002-10-01
期刊：
影响因子：
7.5
通讯作者：
Katoh, R
中科院分区：
文献类型：
--
作者：
Nakamura, N;Miyagi, E;Katoh, R
应用免疫组织化学和原位杂交技术，结合逆转录聚合酶链反应，研究了甲状腺转录因子-1在人肺正常组织和肿瘤组织及细胞系中的表达。在正常肺组织中，肺泡细胞和细支气管细胞核中可见甲状腺转录因子-1的免疫产物。有趣的是，在远端细支气管中，免疫组织化学和原位杂交显示，甲状腺转录因子-1不仅存在于非纤毛细胞（Clara细胞）中，也存在于纤毛细胞和基底细胞中。在肿瘤组织中，甲状腺转录因子-1在腺癌和小细胞肺癌中表达频率较高。分别为96%和89%。甲状腺转录因子-1在鳞状细胞癌和大细胞癌中未检测到。甲状腺转录因子-1的强免疫反应性或甲状腺转录因子-1和表面活性剂蛋白A的同时表达往往与腺癌的分化表型相关，它们在高分化型中比中分化型和/或低分化型中更常见。通过逆转录聚合酶链反应，除腺癌和小细胞肺癌外，甲状腺转录因子-1信使RNA在鳞状细胞癌中也有表达，这一发现在鳞状细胞癌细胞系中得到证实。除肺和甲状腺外的其他器官的99例腺癌中，只有1例免疫组织化学表达甲状腺转录因子-1。我们的研究结果表明，甲状腺转录因子-1在细支气管和肺泡细胞的维持和/或分化过程中发挥重要作用。甲状腺转录因子-1的表达与肺癌的组织学类型和/或分化有关，可以作为更好地了解其生物学性质和病理行为的有价值的标志。
The expression of thyroid transcription factor-1 in normal and neoplastic tissues and cell lines of the human lung was investigated using immunohistochemistry and in situ hybridization in conjunction with reverse transcription polymerase chain reaction. In normal lung tissues, immunoproducts of thyroid transcription factor-1 were observed in the nuclei of alveolar cells and bronchiolar cells. Interestingly, in distal bronchioles, immunohistochemistry and in situ hybridization revealed that thyroid transcription factor-1 was present not only in nonciliated cells (Clara cells) but also in ciliated cells and basal cells. In neoplastic tissues, thyroid transcription factor-1 was demonstrated in adenocarcinomas and small cell lung carcinomas with high frequency. 96% and 89% of cases, respectively. Thyroid transcription factor-1 was not detected in squamous cell carcinomas and large cell carcinomas. The strong immunoreactivity of thyroid transcription factor-1 or simultaneous expressions of thyroid transcription factor-1 and surfactant protein A tended to correlate with the differentiation phenotypes in adenocarcinomas, they were more frequently present in the well-differentiated type than were moderately and/or poorly differentiated types. By reverse transcription polymerase chain reaction, expression of thyroid transcription factor-1 messenger RNA was observed in squamous cell carcinomas in addition to in adenocarcinomas and small cell lung carcinomas, and this finding was confirmed in the cell lines from squamous cell carcinomas. Only one case of 99 adenocarcinomas that originated in various organs other than lung and thyroid immunohistochemically expressed thyroid transcription factor-1. Our results suggest that thyroid transcription factor-1 can play an important role for the maintenance and/or differentiation process in bronchiolar and alveolar cells. Thyroid transcription factor-1 expression associates with histologic types and/or differentiation of lung cancers and can be a valuable marker for the better understanding of their biological nature and pathological behavior.