Electroantennogram and oviposition bioassay responses of Culex quinquefasciatus and Culex tarsalis (Diptera: Culicidae) to chemicals in odors from Bermuda grass infusions
Electroantennogram and oviposition bioassay responses of Culex quinquefasciatus and Culex tarsalis (Diptera: Culicidae) to chemicals in odors from Bermuda grass infusions
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DOI：
10.1093/jmedent/36.2.158
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发表时间：
1999-03-01
影响因子：
2.1
通讯作者：
Millar, JG
中科院分区：
文献类型：
--
作者：
Du, YJ;Millar, JG
气味是从百慕大草发酵浸液上方的顶部空间收集的，百慕大草通常用作诱捕妊娠蚊子的引诱剂。采用气相色谱-触角电图检测 (GC-EAD) 方法从气味提取物中鉴定出 9 种化合物（苯酚、对甲酚、4-乙基苯酚吲哚、3-甲基吲哚、壬醛、2-十一烷酮、2-十三烷酮、萘），这些化合物引起了妊娠雌性致倦库蚊触角的显着触角反应 和跗库蚊。在实验室生物测定中，适当浓度的这些化合物中的几种对怀孕雌性蚊子的吸引力较弱，和/或比水对照刺激更多的产卵。此外，气味提取物的重要成分二甲基三硫醚在 GC-EAD 测定中不会引起雌性蚊子触角的强烈反应，但在 I 浓度下似乎也能刺激产卵。 10 种化合物的重组混合物比单独的化合物产生了更强、更一致的反应。该混合物对怀孕的 Cx、致死鱼具有吸引力，并在跨越 3 个数量级的浓度范围内增强产卵。该混合物的一种浓度也吸引了妊娠铬。跗骨和增强产卵。然而，在测试的最高浓度下，该混合物对两种物种均具有排斥性。总体而言，这些研究表明，怀孕的蚊子被化合物的混合物而不是单一的化学物质吸引到产卵地点，并且气味中化合物的浓度是决定反应是积极的（有吸引力的、刺激性的）还是消极的（驱避性的、威慑性的）的关键因素。
Odors were collected from the headspace above fermented infusions of Bermuda grass, which commonly are used as attractants in traps for gravid mosquitoes. Coupled gas chromatography-electroantennogram detection (GC-EAD) was used to identify 9 compounds (phenol, p-cresol, 4-ethylphenol indole, 3-methylindole, nonanal, 2-undecanone, 2-tridecanone, naphthalene) from odor extracts that elicited significant antennal responses from antennae of gravid female Culex quinquefasciatus Say and Culex tarsalis Coquillett mosquitoes. Several of these compounds at appropriate concentrations were weakly attractive to gravid female mosquitoes in laboratory bioassays and/or stimulated more oviposition than water controls. In addition, dimethyltrisulfide, a significant component of odor extract which did not elicit strong responses fi om female mosquito antennae in GC-EAD assays, also appeared to stimulate oviposition at I concentration. A reconstituted blend of the 10 compounds resulted in much stronger and more consistent responses than individual compounds. The blend was attractive to gravid Cx, quinquefasciatus and enhanced oviposition over a range of concentrations spanning 3 orders of magnitude. One concentration of the blend also attracted gravid Cr. tarsalis and enhanced oviposition. However, at the highest concentration tested, the blend was repellent to both species. Overall, these studies demonstrated that gravid mosquitoes are attracted to oviposition sites by blends of compounds rather than by individual chemicals, and that the concentration of compounds in the odor is a critical factor in determining whether responses are positive (attractive, stimulatory) or negative (repellent, deterrent).