Profiling glucosinolates in vegetative and reproductive tissues of four Brassica species of the U-triangle for their biofumigation potential
Profiling glucosinolates in vegetative and reproductive tissues of four Brassica species of the U-triangle for their biofumigation potential
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DOI：
10.1002/jsfa.2896
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发表时间：
2007-06-01
影响因子：
4.1
通讯作者：
Sorensen, Hilmer
中科院分区：
文献类型：
--
作者：
Bellostas, Natalia;Sorensen, Jens C.;Sorensen, Hilmer
芥子油苷是存在于所有山柑目植物中的氨基酸衍生的化感物质。这些化合物被黑芥子酶同工酶降解，释放出一系列由母体芥子油苷和反应条件确定的生物活性产物。发现紫茎泽兰科内的物种在其硫代葡萄糖苷谱和硫代葡萄糖苷浓度方面不同。同一株植物的不同组织也表现出这种差异，这种差异还受到生长阶段和环境条件的影响。在本文描述的实验中，四个芸苔属物种的U-三角形（B. carinata，B. nigra，B. juncea和B.比较了它们在根、茎、叶和生殖器官不同发育阶段的硫代葡萄糖苷含量。还测定了相应成熟种子的硫代葡萄糖苷谱。丙-2-烯基硫代葡萄糖苷被鉴定为三种植物中的主要硫代葡萄糖苷，其中它占成熟种子中总硫代葡萄糖苷浓度的90%以上和绿色组织中的50%以上。在植物生长过程中，所有组织中硫代葡萄糖苷的相对浓度都增加。芜菁表现出不同的硫代葡萄糖甙谱比三个muclides，具有较高浓度的丁-3-烯基硫代葡萄糖甙，2-羟基丁-3-烯基硫代葡萄糖甙和2-羟基戊-4-烯基硫代葡萄糖甙。B中吲哚-3-基甲基硫代葡萄糖苷的浓度也较高。与芥菜植物相比，芜菁中的硫代葡萄糖苷含量更高，其中4-羟基葡萄糖苷占成熟种子中总硫代葡萄糖苷浓度的30%。总硫代葡萄糖苷浓度的研究物种不同的生长阶段和Muximus达到最大值接近监测期结束。根和叶中硫代葡萄糖苷浓度降低，而生殖组织中硫代葡萄糖苷浓度升高。确定的硫代葡萄糖苷配置文件是在评估的生物熏蒸潜力，这些物种的菊科植物的第一步。(c)2007化学工业协会。
Glucosinolates are amino acid derived allelochemicals present in all plants of the order Capparales. These compounds are degraded by myrosinase isoenzymes, releasing a series of biologically active products defined by the parent glucosinolate and the reaction conditions. Species within the Brassicaceae are found to differ in their glucosinolate profile and glucosinolate concentrations. Different tissues within a single plant also show such variations, which are further influenced by the growth stage and environmental conditions. In the experiments described in this paper, four Brassica species of the U-triangle (B. carinata, B. nigra, B. juncea and B. rapa) were compared with respect to their glucosinolate profiles in roots, stems, leaves and reproductive organs at different developmental stages. The glucosinolate profile of corresponding ripe seeds was also determined. Prop-2-enylglucosinolate was identified as the major glucosinolate in the three mustards, where it represented over 90% of the total glucosinolate concentration of ripe seeds and over 50% of green tissues. The relative concentration of this glucosinolate increased in all tissues during plant growth. Brassica rapa showed a different glucosinolate profile than the three mustards, with higher concentrations of but-3-enylglucosinolate, 2-hydroxybut-3-enylglucosinolate and 2-hydroxypent-4-enylglucosinolate. The concentration of indol-3-ylmethylglucosinolates was also higher in B. rapa than in the mustard plants, with 4-hydroxyglucobrassicin representing 30% of the total glucosinolate concentration in ripe seeds. The total glucosinolate concentration of the species studied varied with growth stage and the mustards achieved a maximum towards the end of the period monitored. Glucosinolate concentration decreased in roots and leaves but increased in reproductive tissues. The determined glucosinolate profiles are an initial step in assessing the biofumigation potential of these species of the Brassicaceae family. (c) 2007 Society of Chemical Industry.