Positive selection from newly formed to marginal zone B cells depends on the rate of clonal production, CD19, and btk
Positive selection from newly formed to marginal zone B cells depends on the rate of clonal production, CD19, and btk
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DOI：
10.1016/s1074-7613(00)80157-0
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发表时间：
2000-01-01
期刊：
影响因子：
32.4
通讯作者：
Kearney, JF
中科院分区：
文献类型：
--
作者：
Martin, F;Kearney, JF
使用免疫球蛋白重链转基因小鼠，我们表明，达到长寿池的B细胞克隆是异质的：一些是在CD 21（高）室（主要是边缘区[MZ]）富集，其他主要驻留在卵泡（FO）。通过N区添加改变B细胞受体的组成降低了克隆产生的速率和MZ富集。这一过程可以重演纯化的CD 21（低）B细胞，是由于一个优先的克隆生存，需要一个功能性的btk酪氨酸激酶，我们还表明，产生的MZ人口是依赖于CD 19。这些发现表明，MZ B细胞库是积极选择的，并具有功能的影响，抗原反应的B细胞从这个微环境。
Using immunoglobulin heavy chain transgenic mice, we show that B cell clones reaching the long-lived pool are heterogeneous: some are enriched in the CD21(high) compartment (mostly marginal zone [MZ]), others reside primarily in the follicles (FO). Altering the composition of the B cell receptor through N region additions decreases the rate of clonal production and the MZ enrichment. This process can be recapitulated by purified CD21(low) B cells and is due to a preferential clonal survival that requires a functional btk tyrosine kinase, We also show that generation of the MZ population is dependent on CD19. These findings suggest that the MZ B cell repertoire is positively selected and have functional implications for antigenic responses effected by B cells from this microenvironment.