Direct force measurement and loading on developing tissues in intact avian embryos
Direct force measurement and loading on developing tissues in intact avian embryos
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完整禽类胚胎发育组织的直接力测量和负载
DOI：
10.1101/2022.06.20.496880
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
L. Mahadevan
中科院分区：
文献类型：
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作者：
Chon U Chan;Fengzhu Xiong;Arthur Michaut;Joana M. N. Vidigueira;O. Pourquié;L. Mahadevan
发育形态发生是由作用于组织流变学的组织应力驱动的。直接测量早期胚胎等小组织 (100μm-1mm) 中的力需要高空间精度和最小侵入性。在这里，我们报告了组织力显微镜（TiFM），它集成了垂直悬臂探针和实时成像，可以对早期鸡胚胎的机械载荷进行闭环控制。通过测试先前定性表征的伸长身体轴中的力产生组织，我们表明 TiFM 能够以高灵敏度定量捕获应力动态。 TiFM 还能够施加稳定、微创和生理相关的负载来驱动组织变形，从而改变形态发生进程和细胞运动。 TiFM 共同解决了小型发育胚胎中组织力测量和操纵的关键技术差距，并有望有助于定量理解发育过程中复杂的多组织力学。
Developmental morphogenesis is driven by tissue stresses acting on tissue rheology. Direct measurements of forces in small tissues (100μm-1mm) in situ such as in early embryos require high spatial precision and minimal invasiveness. Here we report tissue force microscopy (TiFM) integrating a vertical cantilever probe and live imaging to enable close-loop control of mechanical loading in early chicken embryos. By testing previously qualitatively characterized force-producing tissues in the elongating body axis, we show that TiFM quantitatively captures stress dynamics with high sensitivity. TiFM also provides the capacity of applying a stable, minimally-invasive and physiologically relevant load to drive tissue deformation, which alters morphogenetic progression and cell movements. Together, TiFM addresses a key technological gap in tissue force measurement and manipulation in small developing embryos, and promises to contribute to the quantitative understanding of complex multi-tissue mechanics during development.
神经调节机制。
DOI：
10.1385/1-59259-065-9:125
发表时间：
2000
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Schoenwolf,GC;Smith,JL
通讯作者：
Smith,JL