Identification and Expression of the Multidrug and Toxic Compound Extrusion (MATE) Gene Family in Capsicum annuum and Solanum tuberosum.
Identification and Expression of the Multidrug and Toxic Compound Extrusion (MATE) Gene Family in Capsicum annuum and Solanum tuberosum.
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辣椒和马铃薯多药和有毒化合物挤出（MATE）基因家族的鉴定和表达
DOI：
10.3390/plants9111448
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发表时间：
2020-10-27
期刊：
影响因子：
--
通讯作者：
Yu L
中科院分区：
文献类型：
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作者：
Chen Q;Wang L;Liu D;Ma S;Dai Y;Zhang X;Wang Y;Hu T;Xiao M;Zhou Y;Qi H;Xiao S;Yu L
多药和有毒化合物挤出（MATE）蛋白是排出代谢物并参与植物发育和毒素解毒的重要转运蛋白。对茄科中的MATE基因家族知之甚少，该家族包括产生广泛的专门代谢物的物种。本研究对辣椒和马铃薯MATE基因进行了序列分析。我们将所有的MATE基因分为五组，根据它们的系统发育关系和它们的基因和蛋白质结构。此外，我们发现串联重复对辣椒MATE家族的扩展有显著贡献，而串联和片段重复都对马铃薯MATE家族的扩展有贡献，表明MATE在这两个茄科物种中采取了不同的进化路径。ω值分析表明，马铃薯和辣椒MATE基因在进化过程中都经历了纯化选择。此外，共线性分析表明，MATE基因在辣椒和马铃薯之间高度保守。MATE启动子中的顺式元件和MATE表达模式的分析显示，MATE蛋白可能在植物发育的许多阶段起作用，特别是在果实成熟期间，并且当暴露于多种胁迫时，与重复的MATE基因之间存在的功能分化一致。总之，我们的研究结果奠定了辣椒和马铃薯MATE基因家族成员的进一步表征的基础。
Multidrug and Toxic Compound Extrusion (MATE) proteins are essential transporters that extrude metabolites and participate in plant development and the detoxification of toxins. Little is known about the MATE gene family in the Solanaceae, which includes species that produce a broad range of specialized metabolites. Here, we identified and analyzed the complement of MATE genes in pepper (Capsicum annuum) and potato (Solanum tuberosum). We classified all MATE genes into five groups based on their phylogenetic relationships and their gene and protein structures. Moreover, we discovered that tandem duplication contributed significantly to the expansion of the pepper MATE family, while both tandem and segmental duplications contributed to the expansion of the potato MATE family, indicating that MATEs took distinct evolutionary paths in these two Solanaceous species. Analysis of ω values showed that all potato and pepper MATE genes experienced purifying selection during evolution. In addition, collinearity analysis showed that MATE genes were highly conserved between pepper and potato. Analysis of cis-elements in MATE promoters and MATE expression patterns revealed that MATE proteins likely function in many stages of plant development, especially during fruit ripening, and when exposed to multiple stresses, consistent with the existence of functional differentiation between duplicated MATE genes. Together, our results lay the foundation for further characterization of pepper and potato MATE gene family members.