Identification of DNA Signatures Suitable for Use in Development of Real-Time PCR Assays by Whole-Genome Sequence Approaches: Use of Streptococcus pyogenes in a Pilot Study
Identification of DNA Signatures Suitable for Use in Development of Real-Time PCR Assays by Whole-Genome Sequence Approaches: Use of Streptococcus pyogenes in a Pilot Study
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DOI：
10.1128/jcm.01155-12
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发表时间：
2012-08-01
影响因子：
9.4
通讯作者：
Lo, Shyh-Ching
中科院分区：
文献类型：
--
作者：
Hung, Guo-Chiuan;Nagamine, Kenjiro;Lo, Shyh-Ching
使用三层公开软件的逐步计算方法被发现可以有效识别化脓性链球菌的 DNA 特征。 PCR 测试验证，15 个签名衍生引物组中有 9 个可以高特异性检测低至 5 fg 的目标 DNA。所选的特征衍生引物组已成功针对所有 23 个临床分离株进行了评估。该方法很容易适用于设计分子测定法，以快速检测和表征各种病原菌。
A stepwise computational approach using three layers of publicly available software was found to effectively identify DNA signatures for Streptococcus pyogenes. PCR testing validated that 9 out of 15 signature-derived primer sets could detect as low as 5 fg of target DNA with high specificity. The selected signature-derived primer sets were successfully evaluated against all 23 clinical isolates. The approach is readily applicable for designing molecular assays for rapid detection and characterization of various pathogenic bacteria.