FITC and Ru(phen)32+ co-doped silica particles as visualized ratiometric pH indicator.
FITC and Ru(phen)32+ co-doped silica particles as visualized ratiometric pH indicator.
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DOI：
10.1186/1556-276x-6-561
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发表时间：
2011-10-25
影响因子：
--
通讯作者：
Yang W
中科院分区：
文献类型：
--
作者：
Xu J;Sun L;Li J;Liang J;Zhang H;Yang W
评价了异硫氰酸荧光素（FITC）和三（1，10-菲咯啉）钌离子（Ru（phen）32+）共掺杂二氧化硅颗粒作为pH指示剂的性能。pH敏感染料（FITC）和参比染料（Ru（phen）32+）在颗粒中的发射强度比依赖于环境的pH。在365 nm紫外灯下，在单一激发波长下，可以测量出两种染料在不同pH值下的发射强度比的变化，并可以用肉眼观察到。特别地，这种FITC和Ru（phen）32+共掺杂的二氧化硅颗粒被鉴定为对FITC的pKa（6.4）附近的pH显示出高灵敏度，使得它们可能用作用于检测细胞内pH微环境的可视化pH指示剂。
The performance of fluorescein isothiocyanate (FITC) and tris(1, 10-phenanathroline) ruthenium ion (Ru(phen)32+) co-doped silica particles as pH indicator was evaluated. The emission intensity ratios of the pH sensitive dye (FITC) and the reference dye (Ru(phen)32+) in the particles were dependent on pH of the environment. The changes in emission intensity ratios of the two dyes under different pH could be measured under single excitation wavelength and readily visualized by naked eye under a 365-nm UV lamp. In particular, such FITC and Ru(phen)32+ co-doped silica particles were identified to show high sensitivity to pH around the pKa of FITC (6.4), making them be potential useful as visualized pH indicator for detection of intracellular pH micro-circumstance.