THE TRANSFORMING DOMAIN ALONE OF THE LATENT MEMBRANE-PROTEIN OF EPSTEIN-BARR VIRUS IS TOXIC TO CELLS WHEN EXPRESSED AT HIGH-LEVELS
THE TRANSFORMING DOMAIN ALONE OF THE LATENT MEMBRANE-PROTEIN OF EPSTEIN-BARR VIRUS IS TOXIC TO CELLS WHEN EXPRESSED AT HIGH-LEVELS
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DOI：
10.1128/jvi.63.6.2469-2475.1989
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发表时间：
1989-06-01
影响因子：
5.4
通讯作者：
BAICHWAL, VR
中科院分区：
文献类型：
--
作者：
HAMMERSCHMIDT, W;SUGDEN, B;BAICHWAL, VR
一种以前未被认识到的活性与爱泼斯坦-巴尔病毒的BNF-1基因产物有关。该基因编码EB病毒的潜伏膜蛋白。当该基因以高水平表达时，它对所有测试的细胞系都是有毒的，其中包括六种人B淋巴细胞系以及BALB/3 T3、143/EBNA-1和HEp-2细胞。BNLF-1基因先前显示在Rat-1和BALB/3 T3细胞中诱导锚定非依赖性和致瘤性生长。我们在这里证明，只有那些在BNLF-1基因中的突变，在锚定非依赖性生长试验中得分为阳性时，在高水平表达的细胞毒性。因此，可能的是，诱导某些啮齿动物细胞系的锚定非依赖性生长所必需的潜伏膜蛋白的相同活性在高水平表达时也赋予许多细胞系毒性。
A previously unrecognized activity has been associated with the product of the BNF-1 gene of Epstein-Barr virus. This gene encodes that latent membrane protein of Epstein-Barr virus. When the gene was expressed at high levels, it was toxic to all cell lines tested, which included six human B-lymphoid lines as well as BALB/3T3, 143/EBNA-1, and HEp-2 cells. The BNLF-1 gene was previously shown to induce anchorage-independent and tumorigenic growth in Rat-1 and BALB/3T3 cells. We demonstrate here that only those mutations in the BNLF-1 gene that score positively in the anchorage-independent growth assay were cytotoxic when expressed at high levels. It is therefore possible that the same activities of the latent membrane protein that are necessary to induce anchorage-independent growth of some rodent cell lines also confer toxicity to many cell lines when expressed at high levels.