Hematologic effects of stem cell factor in vivo and in vitro in rodents.
Hematologic effects of stem cell factor in vivo and in vitro in rodents.
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DOI：
10.1182/blood.v78.3.645.bloodjournal783645
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发表时间：
1991-08
期刊：
影响因子：
20.3
通讯作者：
T. Ulich;J. Castillo;Eunhee S. Yi;Songmei Yin;Ian McNiece;Yee Pang Yung;K. Zsebo
中科院分区：
文献类型：
--
作者：
T. Ulich;J. Castillo;Eunhee S. Yi;Songmei Yin;Ian McNiece;Yee Pang Yung;K. Zsebo
重组大鼠干细胞因子（rrSCF）以单次静脉注射的方式给予大鼠，会导致剂量依赖性中性粒细胞增多和淋巴细胞增多，以及循环中出现未成熟的骨髓细胞和偶尔的母细胞。中性粒细胞增多在 2 小时开始，在 4 至 6 小时达到高峰，并在 12 至 24 小时之间消退。淋巴细胞增多发生在 0.5 小时，并在 2 小时消退。 rrSCF 诱导的中性粒细胞增多和淋巴细胞增多可通过煮沸消除，这表明 rrSCF 制剂的内毒素污染并不是造成观察到的血液学效应的原因。注射rrSCF后6小时的骨髓显示左移的骨髓和红系增生，形态学上可识别的早期骨髓和红系前体的绝对数量显着增加证明了这一点。注意到成熟骨髓中性粒细胞的绝对数量同时减少，表明骨髓中性粒细胞的释放有助于外周中性粒细胞增多。每天注射 rrSCF 两周后，骨髓涂片显示肥大细胞显着增生，并伴有骨髓细胞总数减少以及明显的红细胞和淋巴组织发育不全。 rrSCF 还会导致肥大细胞出现在循环中，并导致胚胎结缔组织型肥大细胞（而非粘膜型肥大细胞）全身性增加。用 rrSCF 对谱系耗尽的小鼠骨髓细胞进行体外长期培养，导致几乎纯粹的肥大细胞生长。
Recombinant rat stem cell factor (rrSCF) administered to rats as a single intravenous injection causes a dose-dependent neutrophilia and lymphocytosis as well as the appearance of immature myeloid cells and occasional blast cells in the circulation. Neutrophilia begins at 2 hours, peaks at 4 to 6 hours, and subsides between 12 and 24 hours. Lymphocytosis occurs at 0.5 hours and has subsided by 2 hours. rrSCF-induced neutrophilia and lymphocytosis are abrogated by boiling, demonstrating that endotoxin-contamination of the rrSCF preparation is not responsible for the observed hematologic effects. The bone marrow at 6 hours after injection of rrSCF shows a left-shifted myeloid and erythroid hyperplasia as evidenced by significant increases in the absolute numbers of morphologically recognizable early myeloid and erythroid precursors. A concurrent decrease in the absolute numbers of mature marrow neutrophils is noted, suggesting that the release of marrow neutrophils contributes to the peripheral neutrophilia. After 2 weeks of daily injections of rrSCF, bone marrow smears demonstrate a remarkable mast cell hyperplasia accompanied by a decrease in total marrow cellularity and by a striking erythroid and lymphoid hypoplasia. rrSCF also causes mast cells to appear in the circulation and causes a systemic increase in embryonic connective tissue-type, but not mucosal-type, mast cells. In vitro long-term culture of lineage-depleted mouse bone marrow cells with rrSCF results in an almost pure outgrowth of mast cells.