Phylogenetic and Expression Analyses of Cullin Family Members Unveil the Role of PbCUL1.C1 in Pollen Tube Growth Underlying Non-self S-RNase in Pear
Phylogenetic and Expression Analyses of Cullin Family Members Unveil the Role of PbCUL1.C1 in Pollen Tube Growth Underlying Non-self S-RNase in Pear
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Cullin 家族成员的系统发育和表达分析揭示了梨非自体 S-RNase 下 PbCUL1.C1 在花粉管生长中的作用
DOI：
10.1007/s11105-020-01221-2
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发表时间：
2020
影响因子：
2.1
通讯作者：
Zhang Shaoling
中科院分区：
文献类型：
--
作者：
Zhou Ye;Huang Yongdan;Wu Lei;Wang Guoming;Gu Chao;Zhang Shaoling
Cullin（CUL）基因家族是最大的一类RING E3连接酶，在泛素-蛋白酶体系统中发挥重要作用，影响植物的生理活动。然而，对果树中的CUL基因知之甚少。本研究从已测序的7种蔷薇科植物基因组中分离到57个CULs基因，并根据系统发育分析将其分为3个亚科。进化分析表明，多次重复事件在蔷薇科CUL基因家族的扩展中起重要作用。表达谱分析表明，只有4个CUL基因在梨花粉和花粉管中高表达。其中PbCUL1.C1在花粉中的表达量最高，但反义寡核苷酸敲低PbCUL1.C1对花粉管生长无影响。有趣的是，PbCUL1.C1的非自体S-RNase和ASO处理的花粉管比单独的非自体S-RNase处理的花粉管长，这表明PbCUL1.C1在非自体S-RNase的作用下对梨的花粉管生长起着重要的作用。这些结果为进一步研究CUL基因在蔷薇科植物自交不亲和性中的生物学作用提供了依据。
Cullin(CUL) gene family organizes the largest class of RING E3 ligases which play crucial roles in the ubiquitin-proteasome system to affect plant physiological activities. However, little is known about theCULgenes in fruit trees. In this study, 57CULgenes were isolated from the sequenced genomes of sevenRosaceaetrees and were divided into three subfamilies according to phylogenetic analyses. Evolutionary analysis indicated that multiple duplication events play important role in the expansion of theCULgene family inRosaceae. The expression pattern shows that only fourCULgenes were highly expressed in pollen and pollen tube in pear. Of theseCULgenes,PbCUL1.C1presented the highest levels of expression in pollen, but the knockdown ofPbCUL1.C1by antisense oligonucleotide (ASO) did not influence pollen tube growth. Interestingly, the pollen tube treated by both the non-self S-RNase and ASO ofPbCUL1.C1was longer than that treated by unique non-self S-RNase, suggesting that thePbCUL1.C1plays an important role for pollen tube growth underlying non-self S-RNase in pear. These results will be useful for exploring the biological roles ofCULgenes in self-incompatibility in Rosaceae trees.