Prolactin in Ovarian Follicular Fluid Stimulates Endothelial Cell Proliferation
Prolactin in Ovarian Follicular Fluid Stimulates Endothelial Cell Proliferation
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DOI：
10.1159/000231720
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发表时间：
2010-01-01
影响因子：
1.7
通讯作者：
Clapp, Carmen
中科院分区：
文献类型：
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作者：
Castilla, Alejandra;Garcia, Celina;Clapp, Carmen
血管生成对于卵泡的生长和成熟及其向黄体的过渡是必不可少的。除了主要的促血管生成因子，血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF），卵泡液（FF）含有激素催乳素（PRL），这是已知的促进体内血管生成。在这里，我们表明，FF从大卵泡，其中含有两倍的PRL水平的FF从小卵泡，刺激内皮细胞增殖的程度比后者，和免疫中和的PRL阻止FF刺激内皮细胞增殖。值得注意的是，FF增加内皮细胞中短PRL受体亚型和长PRL受体亚型的表达，并且纯化的PRL标准品刺激内皮细胞增殖，但仅在细胞已经用FF预处理之后。然而，纯化的PRL在没有FF预处理的情况下激活内皮细胞中的JAK 2/STAT 3通路。总之，存在于FF中的PRL刺激内皮细胞的增殖。这种效应可能涉及短和长PRL受体亚型的上调，并且不依赖于PRL诱导的JAK 2/STAT 3信号传导。版权所有（C）2009 S. Karger AG，巴塞尔
Angiogenesis is essential for the growth and maturation of the ovarian follicle and its transition into the corpus luteum. In addition to the main proangiogenic factors, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), follicular fluid (FF) contains the hormone prolactin (PRL), which is known to promote angiogenesis in vivo. Here, we show that FF from large follicles, which contains twice the PRL level of FF from small follicles, stimulates endothelial cell proliferation to a greater extent than the latter, and that immunoneutralization of PRL prevents FF from stimulating endothelial cell proliferation. Notably, the FF increases the expression of the short and long PRL receptor isoforms in endothelial cells, and a purified PRL standard stimulates endothelial cell proliferation but only after the cells have been pretreated with FF. However, purified PRL activates the JAK2/STAT3 pathway in endothelial cells in the absence of pretreatment with FF. In summary, PRL present in the FF stimulates the proliferation of endothelial cells. This effect likely involves the upregulation of the short and long PRL receptor isoforms and is independent of PRL-induced JAK2/STAT3 signaling. Copyright (C) 2009 S. Karger AG, Basel