INHIBITION OF GROWTH FACTOR-INDUCED DIFFERENTIATION OF PC12 CELLS BY MICROINJECTION OF ANTIBODY TO RAS P21
INHIBITION OF GROWTH FACTOR-INDUCED DIFFERENTIATION OF PC12 CELLS BY MICROINJECTION OF ANTIBODY TO RAS P21
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DOI：
10.1038/319680a0
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发表时间：
1986-02-20
期刊：
影响因子：
64.8
通讯作者：
VIOLA, M
中科院分区：
文献类型：
--
作者：
HAGAG, N;HALEGOUA, S;VIOLA, M
治疗细胞原癌基因的蛋白产物（p21）被认为可转导诱导细胞分裂所必需的膜信号1 - 4。然而，尚不清楚rasp21在介导配体-膜受体信号导致细胞分化中的确切作用。用神经生长因子（NGF）处理大鼠嗜铬细胞瘤细胞（PC12）可诱导交感神经元的许多表型特征，包括细胞分裂停止和神经元突（神经突）的生长。在这里，我们报告了向PC12细胞中微量注射抗体torasp21抑制了神经突的形成，并导致部分延伸的神经突暂时消退，这种效果在NGF治疗开始后36小时内观察到。注射抗p21抗体对环AMP诱导的神经突形成无影响。这些结果表明，p21参与了ngf诱导的PC12细胞神经突形成的起始阶段，并在激素介导的细胞反应中发挥作用，而不是细胞增殖。
The protein products (p21) of therascellular proto-oncogenes are thought to transduce membrane signals necessary for the induction of cell division1–4. However, there is uncertainty as to the precise role ofrasp21 in mediating ligand-membrane receptor signals leading to cell differentiation. Treatment of rat phaeo-chromocytoma cells (PC12)with nerve growth factor (NGF) results in the induction of a number of phenotypic characteristics of sympathetic neurones, including cessation of cell division and outgrowth of neuronal processes (neurites). Here we report that microinjection of antibody torasp21 into PC12 cells inhibited neurite formation and resulted in temporary regression of partially extended neurites, an effect which was observed up to 36 h after initiation of NGF treatment. Neurite formation induced by cyclic AMP was unaffected by injection of anti-p21 antibody. These results indicate that p21 is involved in the initiation phase of NGF-induced neurite formation in PC12 cells and has a role in hormone-mediated cellular responses distinct from cell proliferation.