Intercellular adhesion molecule-1 (ICAM-1, CD54) is increased in adhesive capsulitis.
Intercellular adhesion molecule-1 (ICAM-1, CD54) is increased in adhesive capsulitis.
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DOI：
10.2106/jbjs.k.00525
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发表时间：
2013-02-20
期刊：
影响因子：
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通讯作者：
Ji, Jong-Hoon
中科院分区：
文献类型：
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作者：
Kim, Yang-Soo;Kim, Jung-Man;Ji, Jong-Hoon
背景技术背景：本研究的目的是探讨细胞间粘附分子-1（ICAM-1）在肩关节粘连性关节囊炎中的表达（“冻结肩”）。方法：盂肱关节囊组织取自26例患者（17例粘连性囊炎和9例对照），并使用寡核苷酸阵列，实时逆转录聚合酶链反应（RT-PCR），和免疫组织ICAM-1也进行了评估滑液中使用蛋白质印迹法（6例粘连性囊炎和两个对照）和外周血中使用酶联免疫吸附试验（ELISA）（32例粘连性囊炎，20例糖尿病，14例对照）。在培养的正常人滑膜细胞中评估ICAM-1处理对与炎症和纤维化相关的细胞因子的基因表达的影响。通过寡核苷酸芯片分析，粘连性关节囊炎患者的盂肱关节囊组织中ICAM-1的水平显著高于对照组（0.12 ± 0.01与0.09 ± 0.00任意单位相比）（p = 0.001）、实时RT-PCR（1.70 ± 0.19与0.67 ± 0.24任意单位相比）（p < 0.05）和免疫组织化学染色。ICAM-1在粘连性关节囊炎患者的滑液中也显著升高（1.70 ± 0.18任意单位）与正常对照组相比（0.48 ± 0.17）粘连性关节囊炎患者血清糖尿病组（671.25 ± 27.08 ng/mL）与对照组（359.86 ± 44.29 ng/mL）比较，差异有统计学意义（p < 0.05）。与未处理的细胞相比，用ICAM-1和用ICAM-1加葡萄糖处理三天后，体外培养的滑膜细胞中与炎症和纤维化相关的细胞因子的基因表达更高。结论：粘连性囊炎患者的ICAM-1增加，类似于糖尿病患者报告的增加。
BACKGROUND: The purpose of this study was to investigate the presence of intercellular adhesion molecule-1 (ICAM-1) in shoulders with adhesive capsulitis ("frozen shoulder").METHODS: Glenohumeral capsular tissue was obtained from twenty-six patients (seventeen with adhesive capsulitis and nine controls), and ICAM-1 was evaluated with use of oligonucleotide arrays, real-time reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemistry. ICAM-1 was also evaluated in synovial fluid with use of western blotting (six patients with adhesive capsulitis and two controls) and in peripheral blood with use of an enzyme-linked immunosorbent assay (ELISA) (thirty-two patients with adhesive capsulitis, twenty with diabetes mellitus, and fourteen controls). The effect of ICAM-1 treatment on gene expression of cytokines related to inflammation and fibrosis was evaluated in cultured normal human synovial cells.RESULTS: The level of ICAM-1 was significantly greater in capsular tissue from the glenohumeral joint of patients with adhesive capsulitis compared with controls as measured by oligonucleotide array analysis (0.12 ± 0.01 compared with 0.09 ± 0.00 arbitrary units) (p = 0.001), real-time RT-PCR (1.70 ± 0.19 compared with 0.67 ± 0.24 arbitrary units) (p < 0.05), and immunohistochemical staining. ICAM-1 was also significantly increased in the synovial fluid of patients with adhesive capsulitis (1.70 ± 0.18 arbitrary units) compared with normal controls (0.48 ± 0.17) (p < 0.05) and in serum of patients with adhesive capsulitis (633.22 ± 59.14 ng/mL) and patients with diabetes mellitus (671.25 ± 27.08 ng/mL) compared with controls (359.86 ± 44.29 ng/mL) (p < 0.05). Gene expression of cytokines related to inflammation and fibrosis in synoviocytes cultured in vitro was greater after three days of treatment with ICAM-1 and with ICAM-1 with glucose compared with untreated cells.CONCLUSIONS: ICAM-1 was increased in patients with adhesive capsulitis, similar to the increase that has been reported in patients with diabetes mellitus.