Development of multiplex PCR method for simultaneous detection of four events of genetically modified maize: DAS-59122-7, MIR604, MON863 and MON88017.
Development of multiplex PCR method for simultaneous detection of four events of genetically modified maize: DAS-59122-7, MIR604, MON863 and MON88017.
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开发多重 PCR 方法同时检测转基因玉米的四个事件：DAS-59122-7、MIR604、MON863 和 MON88017。
DOI：
10.3358/shokueishi.51.92
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
K. Kitta
中科院分区：
文献类型：
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作者：
Taichi Oguchi;Mari Onishi;J. Mano;H. Akiyama;R. Teshima;S. Futo;S. Furui;K. Kitta
建立了一种同时检测转基因玉米DAS-59122-7、MIR604、MON88017和MON863 4个事件特异性事件的多重PCR方法。利用含有不同浓度转基因DNA和非转基因DNA的模拟DNA混合物对分析性能进行单实验室检验，多重PCR方法对MON863、MIR604和MON88017的检出限（LOD）为0.16%，对DAS-59122-7的检出限（LOD）为0.078%。我们之前开发了一种非单plex (9plex) PCR方法，用于8个转基因玉米事件，即Bt11、Bt176、GA21、MON810、MON863、NK603、T25和TC1507。与非单轴PCR方法一起，新开发的方法能够检测和鉴定11个转基因玉米事件，这些事件经常包含在日本使用的商业转基因种子中。此外，该组合分析还可用于转基因玉米组合产物的鉴定。
A novel multiplex PCR method was developed for simultaneous event-specific detection of four events of GM maize, i.e., DAS-59122-7, MIR604, MON88017, and MON863. The single laboratory examination of analytical performance using simulated DNA mixtures containing GM DNA at various concentrations in non-GM DNA suggested that the limits of detection (LOD) of the multiplex PCR method were 0.16% for MON863, MIR604, and MON88017, and 0.078% for DAS-59122-7. We previously developed a nonaplex (9plex) PCR method for eight events of GM maize, i.e., Bt11, Bt176, GA21, MON810, MON863, NK603, T25, and TC1507. Together with the nonaplex PCR method, the newly developed method enabled the detection and identification of eleven GM maize events that are frequently included in commercial GM seed used in Japan. In addition, this combinational analysis may be useful for the identification of combined event products of GM maize.
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DOI：
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发表时间：
2009
期刊：
影响因子：
--
作者：
文津富宏;荒井浩道;石川明人;伊藤智樹;長須正明;稲場圭信;宮本みち子;佐藤恵;稲場圭信;宮本みち子;崎山治男;平塚眞樹;稲場圭信;乾彰男;葛西 賢太;荒井浩道;津富宏;荒井浩道;玉置 充子;津富宏;玉置 充子;井口高志;藤本頼生;上田光明・尾山滋・津富宏;井口高志;藤本頼生;長須正明・下村英雄;三井さよ;菊池結;新谷周平;井口高志;菊池結;樋口明彦;ポール・デュムシエル;井口高志
通讯作者：
井口高志