ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI
ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI
复制标题
DOI：
10.1093/nar/11.5.1475
复制
发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
--
作者：
DIGNAM, JD;LEBOVITZ, RM;ROEDER, RG
我们已经开发了一种程序，用于从人组织培养细胞的细胞核中制备提取物，该提取物在体外指导从II类启动子的准确转录起始。使用腺病毒2的主要晚期启动子优化了提取和测定的条件以获得最大活性。该提取物还指导其他腺病毒启动子和细胞启动子的准确转录起始。该提取物还指导III类启动子（tRNA和Ad 2 VA）的准确转录起始。
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).