Conversion of Dehydrodiferulic Acids by Human Intestinal Microbiota
Conversion of Dehydrodiferulic Acids by Human Intestinal Microbiota
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DOI：
10.1021/jf900159h
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发表时间：
2009-04-22
影响因子：
6.1
通讯作者：
Blaut, Michael
中科院分区：
文献类型：
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作者：
Braune, Annett;Bunzel, Mirko;Blaut, Michael
植物细胞壁相关的脱氢二阿魏酸（DFA）是人类摄入的谷类不溶性膳食纤维的丰富成分。通过将8-O-4和5-5偶联DFA与粪便悬浮液孵育，研究了人肠道微生物群对DFA的体外转化能力。8-O-4-DFA被大多数供体肠道菌群完全降解，主要代谢产物为纯香草酸、3-（3,4-二羟基苯基）丙酸和3,4-二羟基苯基乙酸。阿魏酸和3-（3-羟基-4-甲氧基苯基）丙酮酸的短暂形成表明醚键的初始裂解。与8-O-4-DFA相比，5-5偶联的DFA不会被任何粪便悬浮液裂解成单体。只有侧链被氢化，甲氧基被去甲基化。人肠道菌群对DFA的裂解取决于它们的耦合类型，这可能会影响DFA的生物利用度和DFA偶联纤维在肠道中的可降解性。
Plant cell wall associated dehydrodiferulic acids (DFA) are abundant components of cereal insoluble dietary fibers ingested by humans. The ability of human intestinal microbiota to convert DFA was studied in vitro by incubating 8-O-4- and 5-5-coupled DFA with fecal suspensions. 8-O-4-DFA was completely degraded by the intestinal microbiota of the majority of donors, yielding homovanillic acid, 3-(3,4-dihydroxyphenyl)propionic acid, and 3,4-dihydroxyphenylacetic acid as the main metabolites. The transient formation of ferulic acid and presumably 3-(3-hydroxy-4-methoxyphenyl)pyruvic acid suggests an initial cleavage of the ether bond. In contrast to 8-O-4-DFA, the 5-5-coupled DFA was not cleaved into monomers by any of the fecal suspensions. Only the side chains were hydrogenated and the methoxy groups were demethylated. The cleavage of DFA by human intestinal microbiota, which depended on their coupling type, may affect both the bioavailability of DFA and the degradability of DFA-coupled fiber in the gut.