Wolbachia establishment and invasion in an Aedes aegypti laboratory population
Wolbachia establishment and invasion in an Aedes aegypti laboratory population
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DOI：
10.1126/science.1117607
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发表时间：
2005-10-14
期刊：
影响因子：
56.9
通讯作者：
Dobson, SL
中科院分区：
文献类型：
--
作者：
Xi, ZY;Khoo, CCH;Dobson, SL
为协助控制病媒传播疾病日益加重的负担而提出的一项战略是种群替换，即用疾病传播能力降低的种群替换自然病媒种群。这种策略的一个重要组成部分是驱动系统;它用于将所需的基因型传播到目标田间种群中。内共生的沃尔巴克氏体细菌是潜在的转基因驱动器，但感染不会自然发生在一些重要的蚊子载体中，特别是埃及伊蚊。在这项工作中，在A.埃及伊蚊，并造成高比率的细胞质不相容性（即消除卵孵化）。实验室笼试验证明了wAlbB扩散到A.埃及人群体在未感染的群体与受感染的女性播种后，在七代内达到感染固定。
A proposed strategy to aid in controlling the growing burden of vector-borne disease is population replacement, in which a natural vector population is replaced by a population with a reduced capacity for disease transmission. An important component of such a strategy is the drive system; which serves to spread a desired genotype into the targeted field population. Endosymbiotic Wolbachia. bacteria are potential transgene drivers, but infections do not naturally occur in some important mosquito vectors, notably Aedes aegypti. In this work, stable infections of wAlbB Wolbachia were established in A. aegypti and caused high rates of cytoplasmic incompatibility (that is, elimination of egg hatch). Laboratory cage tests demonstrated the ability of wAlbB to spread into an A. aegypti population after seeding of an uninfected population with infected females, reaching infection fixation within seven generations.