Influence of erythrocyte aggregation on leukocyte margination in postcapillary venules of rat mesentery
Influence of erythrocyte aggregation on leukocyte margination in postcapillary venules of rat mesentery
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DOI：
10.1152/ajpheart.2000.279.4.h1460
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发表时间：
2000-10-01
影响因子：
4.8
通讯作者：
Lipowsky, HH
中科院分区：
文献类型：
--
作者：
Pearson, MJ;Lipowsky, HH
本文用活体显微镜观察了红细胞（RBC）聚集对白细胞（白色细胞; WBC）聚集的影响。光学技术进行了改进，并应用于相关的红细胞的光散射特性，以获得定量指标的聚集体大小（G），这在理想化的条件下，代表每个聚集体的红细胞数量。WBC边缘化，定义为WBC向小静脉壁的径向迁移及其随后沿内皮沿着滚动，测量为微血管腔内潜在最大WBC体积通量（F-WBC*）的百分比。在正常血液中，当壁剪切率（γ/点）从稳态值600降低到稳态值600时，F-WBC* 指数增加4倍，G从1增加到1.15。
The role of erythrocyte (red blood cell; RBC) aggregation in affecting leukocyte (white blood cell; WBC) margination in postcapillary venules of the mesentery (rat) was explored by direct intravital microscopy. Optical techniques were refined and applied to relate the light-scattering properties of RBCs to obtain a quantitative index of aggregate size (G), which, under idealized conditions, represents the number of RBCs per aggregate. WBC margination, defined as the radial migration of WBCs to the venular wall and their subsequent rolling along the endothelium, was measured as the percentage of the potentially maximal WBC volumetric flux within the microvessel lumen (F-WBC*). In normal blood, F-WBC* increased exponentially fourfold, and G increased from 1 to 1.15 as wall shear rates ((gamma) over dot) were reduced from a steady-state value of similar to 600 to