A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay
A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay
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DOI：
10.1093/molehr/2.8.613
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发表时间：
1996-08-01
影响因子：
4
通讯作者：
Thompson, W.
中科院分区：
文献类型：
--
作者：
Hughes, Clara M.;Lewis, Sheena E. M.;Thompson, W.
用改良的碱性单细胞凝胶电泳法比较了生育男性和不育男性精子的基线DNA损伤。研究了正常精子症、正常精子症不育和弱精子症不育(世界卫生组织标准，1992)的精液。三组之间的基线损害水平没有显著差异。在所有样本中，基线损伤的中位数接近20%(80%的头部DNA)。当检查中值时，X射线(5-30Gy射线)照射在可育样本中没有产生额外的损害。然而，30GyX射线照射对这两组不孕症患者都产生了显著的损害。在弱精子症组，过氧化氢(40MU)处理会引起显著的损伤，而在正常精子症可育和不育的样本中，则需要100MUM的双氧水才能引起显著的损伤。在可育群体中，在处理和未处理的样本中都观察到了头部DNA百分比大于80%的亚群。在两组不孕症患者中，这一亚组在治疗后显著降低。我们的结论是，弱精子症不育组比正常精子症不育组更容易受到损害，而正常精子症不育组又比可育组更容易受到损害。可育组包含具有相对完整DNA的抗精子亚群。
Baseline DNA damage in spermatozoa from fertile and infertile men was compared using a modified alkali single cell gel electrophoresis (comet) assay. Semen from normozoospermic fertile, normozoospermic infertile and asthenozoospermic infertile (World Health Organization criteria, 1992) samples were studied. No significant difference was observed in levels of baseline damage between the three groups. A median value for baseline damage of similar to 20% (80% head DNA) was obtained in all samples. Irradiation with X-rays (5-30 Gy) produced no additional damage in fertile samples when median values were examined. However, irradiation with 30 Gy X-rays produced significant damage in both infertile groups. Hydrogen peroxide (40 mu M) treatment induced significant damage in the asthenozoospermic group, whereas 100 mu M H2O2 was required to cause significant damage in the normozoospermic fertile and infertile samples. Within the fertile population a subgroup in which percentage head DNA was greater than 80% was observed in both treated and untreated specimens. This subgroup significantly decreased with treatment in both infertile groups. We conclude that the asthenozoospermic infertile group is more susceptible to damage than the normozoospermic infertile group, which in turn is more susceptible than the fertile group. The fertile group contains a resistant subpopulation of spermatozoa with relatively intact DNA.