Subendothelial smooth muscle cells of human aorta express macrophage antigen in situ and in vitro
Subendothelial smooth muscle cells of human aorta express macrophage antigen in situ and in vitro
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DOI：
10.1016/s0021-9150(97)00136-6
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发表时间：
1997-11-01
期刊：
影响因子：
5.3
通讯作者：
Orekhov, AN
中科院分区：
文献类型：
--
作者：
Andreeva, ER;Pugach, IM;Orekhov, AN
用免疫细胞化学方法对人主动脉内膜内膜进行免疫细胞化学鉴定，发现含有平滑肌细胞标记物α-肌动蛋白和巨噬细胞标记物CD68抗原的细胞。在大体正常的主动脉区域和动脉粥样硬化病变(脂肪条纹和动脉粥样硬化斑块)中，均可检测到表达平滑肌α-肌动蛋白、巨噬细胞CD68抗原和这两种标记的细胞，即具有巨噬细胞抗原的平滑肌细胞。CD68阳性的平滑肌细胞最常见于脂肪丰富的部位：脂肪条纹和动脉粥样硬化的肩部。从大体正常和动脉粥样硬化内膜制备的原代培养细胞中也发现表达平滑肌cc-肌动蛋白和CD68的细胞。在所有检测的培养物中都发现了表达这两种抗原的细胞。在大体正常区域和动脉粥样硬化斑块的培养中，这些细胞的比例相似：分别为14.5+/-4.1%和14.6+/-4.8%。来自脂肪条纹的培养细胞表达这两种抗原的细胞含量较高：25.1+/-7.0%。经修饰的低密度脂蛋白诱导正常内膜细胞内脂质积聚，导致共享CC-肌动蛋白和CD68抗原的细胞数量增加三倍。通过吞噬作用积累乳胶珠也有类似的效果。提示在动脉粥样硬化病变中，细胞内脂质积聚和其他吞噬刺激因子可刺激人主动脉内皮下内膜稳定细胞表达巨噬细胞相关抗原CD68。(C)1997年爱思唯尔爱尔兰科学有限公司。
Cells bearing a smooth muscle cell marker-alpha-actin and a macrophage marker-CD68 antigen were immunocytochemically identified on 'en face' preparations of human aortic intima. Cells, expressing smooth muscle alpha-actin, macrophage CD68 antigen and both markers, i.e. smooth muscle cells possessing the macrophage antigen, were identified both in grossly normal aortic areas and in atherosclerotic lesions (fatty streaks and atherosclerotic plaques). CD68-positive smooth muscle cells were most common in the lipid-rich areas: fatty streaks and atherosclerotic plaque shoulders. Cells expressing smooth muscle cc-actin and CD68 were also revealed in primary cultures prepared from grossly normal and atherosclerotic intima. Cells expressing both antigens were found in all examined cultures. The proportion of these cells in cultures from grossly normal areas and atherosclerotic plaques was similar: 14.5 +/- 4.1 and 14.6 +/- 4.8%, respectively. Cultures from fatty streaks had a higher content of cells expressing both antigens: 25.1 +/- 7.0%. Modified low density lipoprotein-induced intracellular lipid accumulation in cells cultured from grossly normal intima led to a three-fold increase in the number of cells sharing cc-actin and CD68 antigen. Accumulation of latex beads by phagocytosis had a similar effect. It was suggested that in atherosclerotic lesions intracellular lipid accumulation and other stimulators of phagocytosis may provoke the expression of macrophage-associated antigen CD68 in settled cells of the subendothelial intima of human aorta. (C) 1997 Elsevier Science Ireland Ltd.