A dual-channel probe with green and near-infrared fluorescence changes for in vitro and in vivo detection of peroxynitrite
A dual-channel probe with green and near-infrared fluorescence changes for in vitro and in vivo detection of peroxynitrite
复制标题
一种具有绿色和近红外荧光变化的双通道探针，用于体外和体内检测过氧亚硝酸盐
DOI：
10.1016/j.aca.2018.12.021
复制
发表时间：
2019-04-25
影响因子：
6.2
通讯作者：
Feng, Guoqiang
中科院分区：
文献类型：
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作者：
Feng, Shumin;Liu, Dandan;Feng, Guoqiang
本研究以香豆素-半罗丹明杂化物为荧光探针，制备了一种检测过氧亚硝基阴离子的双通道荧光探针。该探针在几乎纯净的水溶液中工作良好，并在两种不同的发射波长（分别为700和515 nm）下对过氧亚硝酸根显示出快速、高灵敏度和选择性的响应，并沿着不同的颜色和绿色到近红外荧光变化。在700和515 nm处的荧光强度比随过氧亚硝酸根浓度从0到50 μ M线性增加，检测限估计为59 nM。此外，在活细胞中的外源性和内源性过氧亚硝酸根的生物成像和在体内检测过氧亚硝酸根与该探针在双荧光通道都成功地应用。结果表明，这种新的探针可以作为一个有用的成像工具，在体外和体内研究过氧亚硝酸根。(C)2018 Elsevier B. V.版权所有。
In this study, a coumarin-semirhodamine hybrid based dual-channel fluorescent probe was developed for detection of peroxynitrite. This probe works well in nearly pure aqueous solution and displays a rapid, highly sensitive and selective response for peroxynitrite at two different emission wavelengths (700 and 515 nm, respectively), along with distinct color and green to near-infrared fluorescence changes. The fluorescent intensity ratio at 700 and 515 nm increases linearly with the concentration of peroxynitrite from 0 to 50 mu M, and the detection limit is estimated to be 59 nM. Moreover, bioimaging of exogenous and endogenous peroxynitrite in living cells and in vivo detection of peroxynitrite with this probe at dual fluorescence channels were all successfully applied. The results suggest that this new probe can be used as a useful imaging tool for studying peroxynitrite in vitro and in vivo. (C) 2018 Elsevier B.V. All rights reserved.