Association analysis of sugar yield-related traits in sorghum [Sorghum bicolor (L.)]
Association analysis of sugar yield-related traits in sorghum [Sorghum bicolor (L.)]
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DOI：
10.1007/s10681-013-0962-7
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发表时间：
2013-10-01
期刊：
影响因子：
1.9
通讯作者：
Liu, Guoqing
中科院分区：
文献类型：
--
作者：
Lv, Peng;Ji, Guisu;Liu, Guoqing
关联作图法是检测高等植物QTL的常用方法。在本研究中，合成高粱人口含有119个代表性的样品，包括43甜和76粒高粱种质资源主要来自中国，美国和印度，使用51个简单重复序列（SSR）标记的基因型。利用TASSEL通用线性模型程序对SSR位点与3个产糖相关性状进行了关联分析。结果表明：（1）同线标记和非同线标记之间存在不同程度的LD，表明高粱连锁群之间存在历史重组，显著的LD可达7.31cM;（iv）4个标记位点与茎秆含糖量相关，鲜茎重和茎汁重在不同生长环境下测量，因此可以用于未来的标记辅助育种计划。几个位点也与两个或两个以上的性状同时，这可能是由于影响这些性状和/或多效性的不同基因之间的紧密连锁。此外，一些相关的标记位于QTL以前定位在家庭为基础的连锁图谱分析。
Association mapping is widely used for detecting QTLs in higher plants. In the present study a synthetic sorghum population containing 119 representative samples, including 43 sweet and 76 grain sorghum accessions originating mainly from China, USA and India, were genotyped using 51 simple-sequence repeat (SSR) markers. Linkage disequilibrium (LD) of pair-wise loci and population structure were analyzed, followed by association analysis of SSR loci and 3 sugar yield related traits using the TASSEL general linear model program. Results showed that: (i) different degrees of LD occurred among syntenic markers and also among nonsyntenic markers, indicating historical recombination among sorghum linkage groups; (ii) significant LD extended up to 7.31 cM; (iii) the collection of accessions was composed of three subgroups; (iv) four marker loci were associated with stalk sugar concentration, fresh stalk weight and stalk juice weight measured in different growing environments and could be used, therefore, in future marker assisted breeding programs. Several loci were also associated with two or more traits simultaneously, which might be due to tight linkage between different genes affecting these traits and/or pleiotropy. In addition, some associated markers were located close to QTLs previously mapped in family-based linkage mapping analyses.