Enhancement of adipose tissue formation by implantation of adipogenic-differentiated preadipocytes
Enhancement of adipose tissue formation by implantation of adipogenic-differentiated preadipocytes
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DOI：
10.1016/j.bbrc.2006.04.089
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发表时间：
2006-06-30
影响因子：
3.1
通讯作者：
Kim, Byung-Soo
中科院分区：
文献类型：
--
作者：
Cho, Seung-Woo;Kim, Inok;Kim, Byung-Soo
工程脂肪组织可用于重建或增强由于塑料和重建手术中乳房切除术或乳房切除术而导致的软组织。前脂肪细胞是脂肪组织再生的可行细胞来源。然而，增强体内脂肪细胞的体内脂肪成分转化仍然是一项主要任务。在体外，植入前的脂肪细胞的脂肪生成分化可能会增强脂肪组织的再生。在本研究中，我们研究了与未分化的预性脂肪细胞相比，脂肪分化的前脂肪细胞的植入是否会增强脂肪组织的形成。我们还研究了碱性成纤维细胞生长因子（BFGF）是否进一步增强了脂肪组织的形成，该脂肪组织的形成是通过脂肪差异分化的前脂肪细胞的植入而介导的。将含有人类前脂肪细胞的纤维蛋白基质与（第1组）或不具有（第2组）BFGF培养的人类前脂肪细胞注入了无胸腺小鼠的皮下空间。还植入了含有（第3组）或没有（第4组）BFGF的未分化的人前叶膜细胞的纤维蛋白基质。植入后六周，植入细胞在所有组中形成新组织。重要的是，与未分化的前脂肪细胞的植入相比，脂肪分化的前胎细胞的植入导致更广泛的脂肪形成，这是通过脂肪组织区域和人类脂肪细胞特异性基因表达评估的，在新形成的新形成的组织中。此外，BFGF增强了新形成的组织中的新血管形成，并进一步增强了由脂肪差异分化的前脂肪细胞介导的脂肪形成。本研究表明，与未分化的前脂肪细胞的植入相比，脂肪分化的前体植入前的前脂肪细胞增强了脂肪组织再生，并且该细胞移植介导的脂肪生成可以通过BFGF的递送来进一步增强。 （c）2006 Elsevier Inc.保留所有权利。
Engineered adipose tissue could be used for the reconstruction or augmentation of soft tissues lost due to mastectomy or lumpectomy in plastic and reconstructive surgery. Preadipocytes are a feasible cell source for adipose tissue regeneration. However, the enhancement of the in vivo adipogenic conversion of preadipocytes remains a major task. In vitro, the adipogenic differentiation of preadipocytes prior to implantation might enhance the adipose tissue regeneration. In the present study, we investigated whether implantation of adipogenic-differentiated preadipocytes enhances the adipose tissue formation compared with implantation of undifferentiated preadipocytes. We also investigated whether basic fibroblast growth factor (bFGF) further enhances the adipose tissue formation mediated by the implantation of adipogenic-differentiated preadipocytes. A fibrin matrix containing human preadipocytes cultured in adipogenic differentiation-inducing conditions with (group 1) or without (group 2) bFGF was injected into the subcutaneous spaces of athymic mice. Fibrin matrices containing undifferentiated human preadipocytes with (group 3) or without (group 4) bFGF were also implanted. Six weeks after implantation, the implanted cells formed new tissues in all groups. Importantly, the implantation of adipogenic-differentiated preadipocytes resulted in more extensive adipogenesis than the implantation of undifferentiated preadipocytes, as evaluated by adipose tissue area and human adipocyte-specific gene expression in the newly formed tissues. In addition, bFGF enhanced neovascularization in the newly formed tissues and further enhanced the adipogenesis mediated by the adipogenic-differentiated preadipocytes. The present study demonstrates that the implantation of adipogenic-differentiated preadipocytes enhances adipose tissue regeneration, as compared with the implantation of undifferentiated preadipocytes, and that cell transplantation-mediated adipogenesis can be further enhanced by the delivery of bFGF. (c) 2006 Elsevier Inc. All rights reserved.