Local photic entrainment of the retinal circadian oscillator in the absence of rods, cones, and melanopsin
Local photic entrainment of the retinal circadian oscillator in the absence of rods, cones, and melanopsin
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DOI：
10.1073/pnas.1323350111
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发表时间：
2014-06-10
影响因子：
11.1
通讯作者：
Van Gelder, Russell N.
中科院分区：
文献类型：
--
作者：
Buhr, Ethan D.;Van Gelder, Russell N.
哺乳动物主昼夜节律起搏器与每日光/暗周期的同步完全通过视网膜光感受器介导。哺乳动物的视网膜本身也是一个自我维持的昼夜节律振荡器。在这里，我们报告了视网膜分子昼夜节律钟可以被体外光照周期所携带，但是杆状细胞、视锥细胞和黑视素（Opn4）并不需要这种携带。在体内，视网膜的Opn4(-/-)；Rd1 / Rd1小鼠与光/暗周期同步，而不管视交叉上核的主昼夜节律起搏器的阶段或动物的行为。这些数据表明，视网膜使用一种单独的机制来局部携带其生物钟，而不是携带生物体水平的节律性。
Synchronization of the mammalian master circadian pacemaker to the daily light/dark cycle is mediated exclusively through retinal photoreceptors. The mammalian retina itself is also a self-sustained circadian oscillator. Here we report that the retinal molecular circadian clock can be entrained by lighting cycles in vitro, but that rods, cones, and melanopsin (Opn4) are not required for this entrainment. In vivo, retinas of Opn4(-/-); rd1/rd1 mice synchronize to light/dark cycles regardless of the phase of the master circadian pacemakers of the suprachiasmatic nuclei or the behavior of the animal. These data demonstrate that the retina uses a separate mechanism for local entrainment of its circadian clock than for entrainment of organism-level rhythmicity.