Adventitious root cultures of Castilleja tenuiflora Benth. as a source of phenylethanoid glycosides
Adventitious root cultures of Castilleja tenuiflora Benth. as a source of phenylethanoid glycosides
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DOI：
10.1016/j.indcrop.2011.09.005
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发表时间：
2012-03-01
影响因子：
5.9
通讯作者：
Trejo-Tapia, Gabriela
中科院分区：
文献类型：
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作者：
Adriana Gomez-Aguirre, Yenny;Zamilpa, Alejandro;Trejo-Tapia, Gabriela
卡斯蒂利亚是一种生长在墨西哥松橡林中的高度珍贵的药用植物。在本研究中，我们首次确定了马鞭草皂苷和异巴豆苷为黄花莲中主要的苯乙醇苷(PhGs)。这些化合物已被证实具有生物活性，包括抗炎、抗氧化和细胞毒活性，这可能与该植物的传统用途有关。我们建立了反相高效液相色谱分析方法，并测定了其在野生植物不同组织中的浓度。马鞭草皂苷主要在根和花序中积累(干生物量分别为9.23和7.88 mg g(-1))，异巴豆苷主要在根中积累(7.13 mg g(-1)干生物量)。为了提供生产生物活性物质的替代原料，我们建立了不定根培养体系，根生长在含有10亩吲哚-3-乙酸(IAA)或10-对-甲基-萘乙酸(NAA)的B5培养基上。根转入含有IAA的培养基后30d，干生物量达到最大值(30g L-1)。在根转移后30d，芦荟皂苷的最大产量为14.62mgg~(-1)干根生物量(438.6 mg L~(-1))，异巴豆苷的最大产量为37.32mgg~(-1)干根生物量(522.48 mg L~(-1))。细花锦鸡儿的不定根培养体系为进一步的放大和苯乙醇苷的生物合成研究提供了良好的系统。(C)2011爱思唯尔B.V.保留所有权利。
Castilleja tenuiflora is a highly valued medicinal plant that grows in pine-oak woods in Mexico. In this study, we identified for the first time verbascoside and isoverbascoside as the major phenylethanoid glycosides (PhGs) in C. tenuiflora. These compounds have proven biological activities, including anti-inflammatory, antioxidant, and cytotoxic activities, which may be related to the traditional uses of this plant. We developed a reverse-phase high-performance liquid chromatography (RP-HPLC) procedure to analyze PhGs, and determined their concentrations in various different tissues of wild plants. Verbascoside accumulated mainly in roots and inflorescences (9.23 and 7.88 mg g(-1) dry biomass, respectively), while isoverbascoside accumulated mainly in the roots (7.13 mg g(-1) dry biomass). To provide an alternative source of material for production of bioactive compounds, we established in vitro adventitious root cultures in which roots were grown in B5 medium containing either 10 mu M indole 3-acetic acid (IAA) or 10 p,M et-naphthaleneacetic acid (NAA). The greatest dry biomass yield (30 g L-1) was achieved at 30 days after transfer of roots into IAA-containing medium. The highest specific yields of PhGs were also obtained using this auxin; the maximum level of verbascoside was 14.62 mg g(-1) dry root biomass (438.6 mg L-1) at 30 days after root transfer, and the maximum yield of isoverbascoside was 37.32 mg g(-1) dry root biomass (522.48 mg L-1) at 23 days after root transfer. Adventitious root cultures of C. tenuiflora are a promising system for further studies on scale-up and phenylethanoid glycosides biosynthesis. (C) 2011 Elsevier B.V. All rights reserved.