The leaf polarity factors SGS3 and YABBYs regulate style elongation through auxin signaling in Mimulus lewisii
The leaf polarity factors SGS3 and YABBYs regulate style elongation through auxin signaling in Mimulus lewisii
复制标题
路易斯酸浆草 (Mimulus lewisii) 中叶片极性因子 SGS3 和 YABBYs 通过生长素信号调节花柱伸长
DOI：
10.1111/nph.17702
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发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Yuan, Yao‐Wu
中科院分区：
文献类型：
--
作者：
Ding, Baoqing;Li, Jingjian;Gurung, Vandana;Lin, Qiaoshan;Sun, Xuemei;Yuan, Yao‐Wu
花柱长度是开花植物育种策略的主要决定因素，在物种之间和物种内可以有很大的差异。然而，对花柱伸长的遗传和发育控制知之甚少。我们研究了SILENCING3(SILENCING3)基因抑制因子和Yabby家族转录因子这两类叶片正轴-背轴极性因子在花柱伸长调控中的作用。我们还研究了花柱发育过程中生长素反应的时空模式。SGS3功能缺失通过限制细胞分裂导致花柱长度缩短，而RNA干扰下调YABBY基因通过减少细胞分裂和细胞伸长导致花柱缩短。我们发现，在雌蕊发育的早期阶段，柱头和子房之间的生长素反应最小，这标志着花柱的分化。随后生长素对该区域的重新分配与花柱伸长相关。当SGS3和YABBY的功能被破坏时，生长素的反应发生了很大的变化。我们认为生长素信号在花柱伸长中起着核心作用，生长素信号控制支持自然花柱长度变异的不同细胞分裂和伸长模式的方式是未来研究的主要问题。
Style length is a major determinant of breeding strategies in flowering plants and can vary dramatically between and within species. However, little is known about the genetic and developmental control of style elongation.We characterized the role of two classes of leaf adaxial–abaxial polarity factors, SUPPRESSOR OF GENE SILENCING3 (SGS3) and the YABBY family transcription factors, in the regulation of style elongation inMimulus lewisii. We also examined the spatiotemporal patterns of auxin response during style development.Loss ofSGS3function led to reduced style length via limiting cell division, and downregulation ofYABBYgenes by RNA interference resulted in shorter styles by decreasing both cell division and cell elongation. We discovered an auxin response minimum between the stigma and ovary during the early stages of pistil development that marks style differentiation. Subsequent redistribution of auxin response to this region was correlated with style elongation. Auxin response was substantially altered when bothSGS3andYABBYfunctions were disrupted.We suggest that auxin signaling plays a central role in style elongation and that the way in which auxin signaling controls the different cell division and elongation patterns underpinning natural style length variation is a major question for future research.