Structure and dynamics of retrotransposons at wheat centromeres and pericentromeres
Structure and dynamics of retrotransposons at wheat centromeres and pericentromeres
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小麦着丝粒和着丝粒周围反转录转座子的结构和动力学
DOI：
10.1007/s00412-008-0161-9
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发表时间：
2008-10-01
期刊：
影响因子：
1.6
通讯作者：
Zhang, Xueyong
中科院分区：
文献类型：
--
作者：
Liu, Zhao;Yue, Wei;Zhang, Xueyong
对多倍体植物中着丝粒DNA的动态变化知之甚少。本文报道了两个来自小麦染色体文库的着丝粒相关细菌人工染色体克隆的序列。自主和非自主小麦着丝粒反转录转座子（CRWs）的鉴定，都是密切相关的小麦着丝粒。纤维荧光原位杂交和染色质免疫沉淀分析表明，小麦着丝粒反转录转座子（CRW）是小麦着丝粒的主要组成部分，与着丝粒功能有关，其拷贝数在不同基因组间存在差异，D基因组染色体的拷贝数少于AorB基因组染色体的拷贝数。长连续CRWarrays的频率高于水稻和玉米。讨论了小麦及其近缘种二倍体形成和多倍体化过程中着丝粒区和近着丝粒区CRW等反转录转座子的动态。
Little is known of the dynamics of centromeric DNA in polyploid plants. We report the sequences of two centromere-associated bacterial artificial chromosome clones from aTriticum boeoticumlibrary. Both autonomous and non-autonomous wheat centromeric retrotransposons (CRWs) were identified, both being closely associated with the centromeres of wheat. Fiber-fluorescence in situ hybridization and chromatin immunoprecipitation analysis showed that wheat centromeric retrotransposons (CRWs) represent a dominant component of the wheat centromere and are associated with centromere function.CRWcopy number showed variation among different genomes: theDgenome chromosomes contained fewer copies than either theAorBgenome chromosomes. The frequency of lengthy continuousCRWarrays was higher than that in either rice or maize. The dynamics ofCRWsand other retrotransposons at centromeric and pericentromeric regions during diploid speciation and polyploidization of wheat and its related species are discussed.