Induction of IL-6 expression by mechanical stress in the trabecular meshwork
Induction of IL-6 expression by mechanical stress in the trabecular meshwork
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DOI：
10.1016/j.bbrc.2005.09.182
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发表时间：
2005-12-02
影响因子：
3.1
通讯作者：
Gonzalez, P
中科院分区：
文献类型：
--
作者：
Liton, PB;Luna, C;Gonzalez, P
小梁网(TM)/施莱姆管（SC）流出通道是维持正常眼压水平的组织。在本研究中，我们研究了机械应力油对TM网络中IL-6表达的影响，以及该细胞因子油流出通路功能的影响。与非应激对照相比，对人TM原代培养物施加循环机械应力导致IL-6的分泌和转录均有统计学意义上的显著增加。tgf - β 1的加入也诱导IL-6的转录和分泌显著激活，据报道，tgf - β 1在机械应力下的TM细胞中上调。此外，抗tgf -b1抗体部分阻断拉伸诱导的IL-6产生。在灌注的猪前段注射IL-6，导致流出设施增加30%，并增加SC细胞单层的通透性。这些结果表明IL-6在房水流出阻力的稳态调节中起作用。(c) 2005爱思唯尔公司版权所有。
The trabecular meshwork (TM)/Schlemm's canal (SC) outflow pathway is the tissue responsible for maintaining normal levels of intraocular pressure. In the present study, we investigate the effects of mechanical stress oil the expression of IL-6 in the TM meshwork,, as well as the effects of this cytokine oil outflow pathway function. Application of cyclic mechanical stress to human TM primary cultures resulted in a statistically significant increase in both secretion and transcription of IL-6, compared to nonstressed controls. Addition of TGF-beta 1, which has been reported to be upregulated in TM cells under mechanical stress, also induced a significant activation of both the transcription and secretion of IL-6. Moreover, anti-TGF-b1 antibodies partially blocked the stretch-induced IL-6 production. Injection of IL-6 into perfused porcine anterior segments resulted in a 30% increase in outflow facility, as well as increased permeability through SC cell monolayers. These results suggest a role for IL-6 in the homeostatic modulation of aqueous humor outflow resistance. (c) 2005 Elsevier Inc. All rights reserved.