The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
复制标题
DOI：
10.3791/51445
复制
发表时间：
2014-03-01
影响因子：
1.2
通讯作者：
Calder, Philip C.
中科院分区：
文献类型：
--
作者：
Fisk, Helena L.;West, Annette L.;Calder, Philip C.
气相色谱法（GC）是一种高灵敏度的方法，用于鉴别和定量组织、细胞和血浆/血清中脂质的脂肪酸含量，产生的结果具有高准确度和高重现性。在代谢和营养研究中，GC可以评估干预后或生理状态变化（如妊娠）期间脂肪酸浓度的变化。使用氨丙基硅胶柱的固相萃取（SPE）可分离主要脂质类，包括三酰甘油、不同磷脂和胆固醇酯（CE）。采用气相色谱-固相萃取法分析了不同高脂和低脂饲料喂养的未孕和孕鼠肝脏CE组分脂肪酸组成的变化。有显着的饮食/妊娠相互作用的影响，肝脏CE的ω-3和ω-6脂肪酸含量，表明怀孕的女性有一个不同的反应，饮食操纵比处女女性。
Gas chromatography (GC) is a highly sensitive method used to identify and quantify the fatty acid content of lipids from tissues, cells, and plasma/serum, yielding results with high accuracy and high reproducibility. In metabolic and nutrition studies GC allows assessment of changes in fatty acid concentrations following interventions or during changes in physiological state such as pregnancy. Solid phase extraction (SPE) using aminopropyl silica cartridges allows separation of the major lipid classes including triacylglycerols, different phospholipids, and cholesteryl esters (CE). GC combined with SPE was used to analyze the changes in fatty acid composition of the CE fraction in the livers of virgin and pregnant rats that had been fed various high and low fat diets. There are significant diet/pregnancy interaction effects upon the omega-3 and omega-6 fatty acid content of liver CE, indicating that pregnant females have a different response to dietary manipulation than is seen among virgin females.