RIBONUCLEOPROTEIN AND PROTEIN FACTORS BIND TO AN H-DNA-FORMING C-MYC DNA ELEMENT - POSSIBLE REGULATORS OF THE C-MYC GENE
RIBONUCLEOPROTEIN AND PROTEIN FACTORS BIND TO AN H-DNA-FORMING C-MYC DNA ELEMENT - POSSIBLE REGULATORS OF THE C-MYC GENE
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DOI：
10.1073/pnas.86.24.9682
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发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
KINNIBURGH, AJ
中科院分区：
文献类型：
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作者：
DAVIS, TL;FIRULLI, AB;KINNIBURGH, AJ
我们已经在c-myc基因的5“侧翼DNA（~ 125个碱基对）内定位了一个正的顺式作用DNA序列元件。该DNA序列元件具有大的嘌呤-嘧啶链不对称性，并且可以呈现H-DNA构象。具有核糖核蛋白（RNP）性质的因子与DNA区域相互作用。c-myc DNA序列元件和RNP的相互作用涉及RNase H敏感机制，因此可能涉及RNA-DNA杂合体。此外，蛋白质因子与该DNA序列元件结合。DNA足迹法和突变体寡核苷酸结合/竞争测定暗示了RNP因子的点状聚（G.cntdot.C）识别/结合序列，而主要蛋白因子需要两个ACCCT序列基序以实现最大结合。这些结果表明，RNP和蛋白质因子作为c-myc基因的正转录调节因子，可能通过改变DNA拓扑结构。
We have located a positive, cis-acting DNA sequence element within the 5'' flanking DNA of the c-myc gene (-125 base pairs). This DNA sequence element has a large purine-pyrimidine strand asymmetry and can assume the H-DNA conformation. A factor with the properties of a ribonucleoprotein (RNP) interacts with the DNA region. The interaction of the c-myc DNA sequence element and the RNP involves an RNase H-sensitive mechanism and, therefore, may involve an RNA-DNA hybrid. In addition, a protein factor(s) binds to this DNA sequence element. DNA footprinting and mutant oligonucleotide binding/competition assays implicate a punctate, poly(G.cntdot.C) recognition/binding sequence for the RNP factor, whereas the major protein factor requires two ACCCT sequence motifs for maximal binding. These results suggest that RNP and protein factors act as positive transcriptional regulators of the c-myc gene, perhaps by altering DNA topology.