Identification of HTA as a novel-specific marker for human hepatocellular carcinoma
Identification of HTA as a novel-specific marker for human hepatocellular carcinoma
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DOI：
10.1007/s00432-010-0767-1
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发表时间：
2010-08-01
影响因子：
3.6
通讯作者：
Li, Guancheng
中科院分区：
文献类型：
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作者：
Liu, Yan;Li, Yuehui;Li, Guancheng
背景 肝细胞癌（HCC）是世界上最常见的恶性肿瘤，尤其是在中国。新发和复发性肝细胞癌的早期诊断并及时治疗将有助于降低死亡率。目前生物标志物并不令人满意。迫切需要更好的诊断方法。方法在本研究中，我们通过计算机模拟鉴定和RT-PCR检测，发现了肝癌相关基因(HTA)。通过恶性肝细胞 HepG2 中的小干扰 RNA 敲低内源性 HTA 表达。然后我们测试了这些细胞的体外和体内增殖能力。结果HTA在某些肿瘤中特异表达，但在任何正常组织中均未检测到。它在肝细胞癌中表达尤其高。通过小干扰 RNA 敲低 HepG2 中的内源性 HTA 表达可减弱 HCC 细胞的生长。结论 HCA 是一种非常好的肿瘤标志物，尤其是 HCC。它可能在 HCC 的发生和进展中发挥重要作用，并且可能成为开发 HCC 新诊断和治疗策略的有前途的分子靶点。
Background Hepatocellular carcinoma (HCC) is the most common malignancy in the world, especially in China. Early diagnosis of new and recurrent hepatocellular carcinoma, followed by timely treatment, will help decrease mortality. Currently biomarkers are not satisfactory. Better diagnostic methods are highly demanded.Methods In this study, we have used in silico identification and RT-PCR test and discovered a hepatoma associated gene (HTA). Knockdown of endogenous HTA expression was performed by small interfering RNA in malignant hepatocyte HepG2. Then we tested the cell proliferative ability of these cells in vitro and in vivo.Results HTA was expressed specifically in some kinds of tumors, but not detected in any normal tissues. It was expressed especially high in hepatocellular carcinoma. Knockdown of endogenous HTA expression in HepG2 by small interfering RNA attenuated HCC cell growth.Conclusions HCA is a very good marker for tumors, especially for HCC. It could play important roles in HCC development and progression and can be a promising molecular target for the development of new diagnostic and therapeutic strategies for HCC.